| 石蜡包埋组织切片原位核酸杂交研究 |
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| 引用本文： | 任立群,赵志涛,孙波,李广生,朴松旭. 石蜡包埋组织切片原位核酸杂交研究[J]. 吉林大学学报(医学版), 2001, 27(3): 235-237 |
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| 作者姓名： | 任立群 赵志涛 孙波 李广生 朴松旭 |
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| 作者单位： | 1. 吉林大学生物工程研究所病理研究室
2. 延边大学附属医院中心实验室 |
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| 基金项目： | 国家自然科学基金;39870668; |
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| 摘 要： | 目的 :探讨石蜡包埋组织切片的原位核酸杂交方法 ,以检测克山病 ( KSD)尸检心肌中的RNA。方法 :应用生物素标记的人工合成寡核苷酸探针 ,对 72例心肌石蜡包埋组织切片进行原位核酸杂交。结果 :各型克山病心肌石蜡组织切片的阳性杂交信号为 80 %～ 87.9% ,而正常对照仅为 14.3%。结论 :应用人工合成寡核苷酸之原位核酸杂交技术 ,对长期保存的石蜡组织进行回顾性研究是可行的。
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| 关 键 词： | 原位核酸杂交 寡核苷酸探针 石蜡包埋组织 |
| 文章编号： | 0253-3707(2001)03-0235-03 |
| 修稿时间： | 2000-06-19 |
Study of hybridization in situ in paraffin-embedded myocardial specimens |
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| REN Li qun,ZHAO Zhi tao,SUN Bo,LI Guang sheng,PIAO Song xu. Study of hybridization in situ in paraffin-embedded myocardial specimens[J]. Journal of Jilin University: Med Ed, 2001, 27(3): 235-237 |
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| Authors: | REN Li qun ZHAO Zhi tao SUN Bo LI Guang sheng PIAO Song xu |
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| Affiliation: | REN Li qun,ZHAO Zhi tao,SUN Bo,LI Guang sheng,PIAO Song xu * |
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| Abstract: | Objective: To explore the method of hybridization in situ in paraffin embedded myocardial specimens in order to examine RNA of Coxsackie virus of myocardial tissue from patients with Keshan disease(KSD). Methods: Hybridization in situ was performed by the use of the oligonucleotide probe by artificial synthesis labelled with biotin.Results:Positive hybrid signal was found in paraffin embedded myocardial specimens. The positive rates in acute,subacute and chronic KSD were 83%(10/12),87.9%(29/33) and 80%(16/20) respectively, but only 14.3%(1/7) in control.Conclusion: It is feasible to perform the reviewable study on paraffin embedded tissue kept for long term using hybridization in situ technology. [ |
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| Keywords: | hybridization in situ oligonucleotide probe paraffin embedded tissue |
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