胎儿卵巢组织移植治疗卵巢性闭经的研究

目的:评价胎儿卵巢组织移植治疗卵巢性闭经的价值。方法:收集孕20周~24周治疗性引产女性胎儿标本,获取卵巢,经程序冷冻后保存,使用前快速复温,加入RPM1640培养液进行组织培养。2003年3月至2003年8月共有3例自愿受试者在我院接受了胎儿卵巢组织移植术,其中1例为卵巢功能不良,1例为先天性卵巢发育不全,另1例为双侧卵巢切除术。结果:胎儿卵巢组织冻存后快速复温,伊红染料排斥试验检测活细胞率可达到84.5%;组织培养后卵原细胞有成熟表现,部分生长为初级卵泡。卵巢组织移植后,2例有自体卵巢患者有周期性月经来潮,其中1例术后第4个月可观察到规则排卵。1例双侧卵巢切除患者潮热症状改善,雌激素水平上升,但不能达到生育年龄水平,月经周期未能恢复。结论:胎儿卵巢组织移植可有效的治疗卵巢内分泌功能不足。

Evaluation of the Human Fetal Ovarian Tissue Transplantation in Patients with Ovarian Amenorrhea

OBJECTIVE:To assess the effects of fetal ovarian tissue transplantation on ovarian amenorrhea.METHODS:Fetal ovary of theraputic abortion at 20 to 24 weeks of gestation was collected,which was saved by procedural fall temperature technique and cultured in RPM1640 after freeze-thawing.From January 2003 to August 2003,three cases were subjected to fetal ovarian tissue transplantation in our hospital.The indication included ovarian dysfunction(1 cases),ovarian dysgenesis(1 case) and bilateral salpingo-oophorectomy(1 case).RESULTS:The rate of living cells of the ovarian tissue was 85.4% after freezing preservation.The processes of germ cellular growth and development was observed in culture.Part of oogonia grew up primary follicles.After transplantation,the menstrual cycle recoved in 2 patients with(auto-ovary) and regularity ovulation was viewed in 1 cases of them.The menstrual cycle did not recove in the patient of bilateral salpingo-oophorectomy,but the level of E_2 was higher than before transplantation and the quality of life was significantly improved.CONCLUSION:Fetal ovarian tissue transplantation is an effective treatment for patients with ovarian amenorrhea.