iNOS和MMP－2在形觉剥夺性近视中的表达变化

目的探讨诱导型一氧化氮合酶（iNOS）和基质金属蛋白酶－2（MMP－2）在形觉剥夺性近视豚鼠眼后极部的表达。方法对2周龄豚鼠以半透明眼罩遮盖右眼9周制备形觉剥夺性近视（FDM）动物模型，左眼作为对照。遮盖3、6、9周后随机抽取15只豚鼠，检影验光检测双眼屈光度，A型超声测量双眼眼轴长度，免疫组织化学检测眼后极部iNOS和MMP-2的表达变化。结果形觉剥夺使豚鼠眼轴延长，近视屈光度增加，遮盖眼与对照眼屈光度和眼轴长差异均有统计学意义（P〈0．05）。遮盖眼iNOS和MMP-2免疫活性逐渐增加，与对照组比较差异有统计学意义（P〈0．05），两者的表达量在形觉剥夺过程中呈强相关性（r=0．769，P〈0．05）。结论iNOS和MMP-2可能参与形觉剥夺性近视的形成，NO信号通路可通过调节巩膜MMP-2的活性造成巩膜重塑。

Expression of iNOS and MMP - 2 in posterior segment of guinea pig eyes with form deprivation myopia

Objective To investigate the changes of inducible nitric oxide synthase （iNOS） and matrix metalloproteinase - 2 （ MMP - 2 ） in posterior segment of guinea pig eyes with form deprivation myopia （FDM）. Methods FDM were established in 2 - week - old guinea pigs whose right eyes were closed with translucent goggles for 9 weeks, the left eyes served as control group. Fifteen guinea pigs were ran- domly selected for retinoscopy and A - scan after 3,6,9 weeks. The expression of retina iNOS and sclera MMP -2 were analyzed by immunohistochemistry. Results The myopia diopter and axial length in FDM eyes were significantly increased in comparison With the control eyes （ P 〈 0.05）. The expression of iN- OS and MMP - 2 in FDM eyes were obviously upregulated compared with control eyes （ P 〈 0.05 ）, A strong correlation between the expression of iNOS and MMP - 2 was detected too. Conclusions iNOS and MMP - 2 may be involved in the regulation of FDM, NO signaling pathway might affect sclera remodeling by regulating MMP -2 activity.