| HPLC法测定少阳感冒颗粒中黄芩苷的含量 |
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| 引用本文： | 杨小月.HPLC法测定少阳感冒颗粒中黄芩苷的含量[J].中国药师,2010,13(2):228-229. |
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| 作者姓名： | 杨小月 |
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| 作者单位： | 怀化市药品检验所,湖南怀化,418000 |
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| 摘 要： | 目的：建立HPLC法测定少阳感冒颗粒中黄芩苷的含量。方法：采用DiamonsilC18柱（250mm×4．6mm，5μm）；以甲醇-水-冰醋酸（50：50：1）为流动相，流速为1．0ml·min-1；检测波长为274nln。结果黄芩苷进样量在0．05～0．28μg范围内线性关系良好，r=0．9997，平均加样回收率为98．0％，RSD为0．82％（n=6）。结论：方法简便易行、准确、重复性好，可用于少阳感冒颗粒的质量控制。
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| 关 键 词： | 高效液相色谱 少阳感冒颗粒 黄芩苷 含量测定 |
Determination of Baicalin in Shaoyang Ganmao Granules by HPLC |
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| Yang Xiaoyue.Determination of Baicalin in Shaoyang Ganmao Granules by HPLC[J].China Pharmacist,2010,13(2):228-229. |
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| Authors: | Yang Xiaoyue |
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| Institution: | Yang Xiaoyue (Huaihua Institute for Drug Control, Hunan, HuaiHua 418000 ,China) |
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| Abstract: | Objective： To establish an HPLC method for the determination of baicalin in shaoyang ganmao granules. Method： A Diamonsil C18 column （250 mm ×4.6 mm,5 μm） was used,the methanol-water-glacial acetic acid （50： 50：1 ） as mobile phase,the flow rate was 1.0 ml.min-1 ;the detection wavelengh was 274 nm. Result： A good linear was obtained within the range of 0.05-0. 28 μg, r = 0. 999 7. the average recovery was 98. 0% （ RSD = 0. 82% , n = 6 ）. Conclusion： The mothod is simple, accurate and repeatable,it can be used for the quality control of shaoyang ganmao granules. |
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| Keywords: | HPLC Shaoyang ganmao granules Baicalin Content determination |
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