|
|||||
|
|
| 抗菌肽PR39真核表达载体的构建及其在巨噬细胞中的表达和抗菌作用 | |
| 引用本文： | 文阳安,郭金英,余晓林,岑东,陈彦,张键,罗淼,涂植光.抗菌肽PR39真核表达载体的构建及其在巨噬细胞中的表达和抗菌作用[J].第三军医大学学报,2008,30(21):2044-2046. |
| 作者姓名： | 文阳安 郭金英 余晓林 岑东 陈彦 张键 罗淼 涂植光 |
| 作者单位： | 1. 重庆医科大学:医学检验系生物化学教研室,临床检验诊断学省部共建教育部重点实验室,重庆市重点实验室 2. 西南大学医院,重庆,400715 3. 重庆医科大学:附属第一医院骨科,重庆,400016 |
| 摘 要： | 目的 构建抗菌肽PR39的真核表达载体,转染后观察PR39在小鼠巨噬细胞RAW264.7中的表达及抗菌作用.方法 采用拼接PCR法合成抗菌肽PR39基因,克隆到真核表达载体pIRES2-GFP中,经鉴定后用阳离子脂质体转染小鼠巨噬细胞RAW264.7,通过RT-PCR和免疫荧光法检测目的 基因的表达,并对其抗菌活性进行初步检测.结果 成功构建了抗菌肽PR39的真核表达载体pIRES2-GFP/PR39,并能在小鼠巨噬细胞RAW264.7中表达抗菌肽PR39.表达抗菌肽PR39的巨噬细胞杀灭和清除胞内菌的能力明显增强.结论 抗菌肽PR39基因能够在小鼠巨噬细胞RAW264.7中表达并发挥抗菌作用. |
| 关 键 词： | 抗菌肽 真核表达载体 巨噬细胞 胞内菌 |
Construction of eukaryotic expression vector encoding antimicrobial peptides PR39 and its expression and antimicrobial function in RAW264.7 cells |
|
| WEN Yang-an,GUO Jin-ying,YU Xiao-lin,CEN Dong,CHEN Yan,ZHANG Jian,LUO Miao,TU Zhi-guang.Construction of eukaryotic expression vector encoding antimicrobial peptides PR39 and its expression and antimicrobial function in RAW264.7 cells[J].Acta Academiae Medicinae Militaris Tertiae,2008,30(21):2044-2046. | |
| Authors: | WEN Yang-an GUO Jin-ying YU Xiao-lin CEN Dong CHEN Yan ZHANG Jian LUO Miao TU Zhi-guang |
| Institution: | WEN Yang-an1,GUO Jin-ying1,YU Xiao-lin1,CEN Dong1,CHEN Yan2,ZHANG Jian3,LUO Miao1,TU Zhi-guang1 (1Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education,Faculty of Laboratory Medicine,Chongqing Medical University,Chongqing 400016,2Infirmary of Southwest University,Chongqing 400715,3Department of Orthopedics,First Affiliated Hospital,China) |
| Abstract: | Objective To construct an eukaryotic expression vector encoding antimicrobial peptide PR39,and observe its expression and antimicrobial function in mouse macrophage RAW264.7 cells.Methods PR39 gene sequence was synthesized with PCR amplification and inserted into an eukaryotic expression vector pIRES2-GFP.After identification,pIRES2-GFP/PR39 vector was transfected into RAW264.7 cells with cationic liposome.The expression of PR39 was detected by RT-PCR and immunofluorescence method,and its antibacterial acti... |
| Keywords: | PR39 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |