蒺藜皂苷对缺血再灌注损伤心肌细胞的保护作用

目的　研究蒺藜皂苷 (GSTT)对缺血再灌注损伤心肌细胞的保护作用。方法　通过模拟缺血再灌注模型 ,研究GSTT对再灌注后LDH、CK漏出量 ,MDA含量 ,SOD活性 ,以及TNF α和IL 6分泌量 ;用浓度为 1 6 2 5ng·L-1 TNF α损伤心肌细胞 ,研究GSTT对培养液中NO生成量的影响 ,并用MTT法检测GSTT对TNF α损伤心肌细胞线粒体酶的保护作用。结果　GSTT可抑制再灌注后LDH、CK漏出量 ,减少MDA含量 ,升高SOD活性 ,抑制TNF α和IL 6的分泌量 ;减少TNF α引起NO生成量 ,升高OD490 吸光度值。结论　GSTT可减轻缺血再灌注对心肌细胞的损伤程度 ,对心肌细胞具有直接的保护作用。

Protective effect of GSTT on myocardium injuried by ischemia reperfusion

AIM To investigate the protective effect of GSTT on myocardial injury induced by ischemia reperfusion. METHODS The content of lactic dehydrogenase(LDH),creatine kinase(CK),methylenedioxyamphetamine(MDA),superoxide dismutase(SOD),tumor necrosis factor alpha(TNF α) and interleukine 6(IL 6) were determined in medium after reperfusion by simulating model of ischemia reperfusion; The content of nitric oxide(NO) was determined in medium by TNF α injury myocardium,and mitochondrial activity was determined by MTT after TNF α stimulated. RESULTS Compared with the model control,in the GSTT, the content of LDH,CK,MDA,TNF α and IL 6 in medium decreased and activity of SOD increased after reperfusoin,the level of NO in medium decreased and OD 490 increased after TNF α injuried. CONCLUSION It is suggested that GSTT can mitigate injury of ischemia reperfusion on myocardium, and has a direct protective effect on injuried myocardium.