子宫颈癌外周血T淋巴细胞亚群检测及其临床意义

[目的]探讨子宫颈癌患者细胞免疫功能的变化及其临床意义.[方法]应用间接免疫荧光染色法检测40例子宫颈癌患者外周血T淋巴细胞亚群的分布及天然杀伤细胞(NK细胞)活性.『结果]子宫颈癌患者外周血T淋巴细胞中的CD3 、CD4 比正常人显著降低(P＜0.001),CD8 比正常人显著升高(P＜0.001),CD4 /CD8 比值比正常人显著降低(P＜0.001).老龄子宫颈癌CD4 下降较年轻病例明显(P＜0.05).子宫颈深层浸润病例CD8 上升,NK细胞百分率下降(P＜0.05).免疫功能抑制与病理类型、分化等其他病理因素无关.[结论]子宫颈癌患者的细胞免疫功能严重受阻,CD8 和NK指标可作为浸润性子宫颈癌的指标.

Detection of T Cell Subsets in Peripheral Blood of Cervical Cancer and its Clinical Significance

To investigate the changes of cell immune function and its clinical significance in patients with cervical cancer. T cell subsets and natural kill cell activity in peripheral blood were detected by indirect immunofluorescent staining in 40 cases with cervical cancer. The proportion of CD3+, CD4+, and the CD4+/CD8+ ratio decreased, while CD8+ increased significantly in comparison with normal control(P<0.001). CD4+ decreased significantly in older patients than that in younger ones(P<0.05). CD8+ subset increased significantly in patients with cervical deep invasion(P<0.05). The alternations of cell immune functions were not related to the histological type, grade and other pathologic factors. [Conclusion] Cell immune function in peripheral blood is highly impaired in patients with cervical cancer. CD8+ and NK may serves as markers for invasive cervical cancer.