Increased rate of DNA recovery from United Kingdom epidemic Clostridium difficile PCR ribotype 1 strains stored cryogenically |
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Authors: | Asha N J Fawley W N Freeman J Wilcox M H |
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Affiliation: | Department of Microbiology, Leeds General Infirmary, The Old Medical School, Thoresby Place, Leeds LS1 3EX, United Kingdom. |
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Abstract: | We noted that some Clostridium difficile isolates are nonrecoverable after frozen storage and so used molecular typing analysis to characterize DNA from these strains. The recovery rate of C. difficile PCR ribotype 1 was statistically significantly greater than that of other strains. This observation has implications for C. difficile epidemiological studies. |
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