MMP-2和TIMP-2在甲状腺乳头状癌中的表达及与转移的关系

目的探讨基质金属蛋白酶MMP-2和金属蛋白酶组织抑制剂TIMP-2在甲状腺乳头状癌中的表达及其与甲状腺乳头状癌侵袭与转移的关系.方法应用原位杂交技术检测95例甲状腺乳头状癌及59例癌旁组织中MMP-2 mRNA,TIMP-2mRNA的表达水平.用免疫组化S-P法检测上述组织中MMP-2蛋白、TIMP-2蛋白的表达.结果MMP-2mRNA和蛋白在甲状腺乳头状癌中的阳性率分别为74.7%和77.9%,而在癌旁组织中的阳性率分别为10.2%和25.4%,两者差异有显著性(P＜0.01,P＜0.01).TIMP-2 mRNA和蛋白在甲状腺乳头状癌中的阳性率分别为58.9%和64.29%,而在癌旁组织中的阳性率分别为20.3%和16.9%,两者差异有显著性(P＜001,P＜0.01).结论基质金属蛋白酶MMP-2mRNA和蛋白、TIMP-2mRNA和蛋白在甲状腺乳头状癌中呈高表达,MMP-2mRNA和蛋白的表达随甲状腺癌侵袭程度升高、转移而升高,而TIMP-2 mRNA和蛋白的表达随甲状腺癌侵袭程度升高、转移而降低,提示MMP-2和TIMP-2表达失衡与甲状腺乳头状癌侵袭与转移有密切关系.

Relationship of MMP-2 and TIMP-2 expression with invasion and metastasis in papillary thyroid carcinoma

Objective: To study the expression of MMP-2 and TIMP-2 and to understand their relationship with tumor invasion and lymph node metastasis in papillary thyroid carcinoma (PTC). Methods: In situ hybridization was used to detect MMP-2 and TIMP-2 mRNA in PTC (n=95) and nearby no-tumorous tissues(n=59). The protein expression of MMP-2 and TIMP-2 in basement membrane was also studied with immunohistochemistry (S-P method). Results: The positive expression rate of MMP-2 mRNA and protein in PTC was 74.7% and 77.9%, respectively; but in nearby no-tumorous tissues, it was only 10.2% and 25.4%.The positive expression rate and intensity in PTC were much higher than that in nearby no-tumorous tissues (P<0.01, P<0.01). The positive expression rate of TIMP-2 mRNA and protein in PTC was 58.9% and 64.2%, respectively; but in nearby no-tumorous tissues, it was only 20.3% and 16.9%, The positive expression rate and intensity in PTC were much higher than that in nearby no-tumorous tissues (P<0.01, P<0.01). Conclusions: MMP-2 mRNA and protein,TIMP-2 mRNA and protein in PTC are highly expressed,the positive expression of MMP-2 has a rising tendency with the advancing invasion. On the contrary, the positive expression of TIMP-2 has a decreasing tendency with the advancing invasion, suggesting that the imbalance between MMP-2 and TIMP-2 is associated with the advancing invasion of PTC.