大鼠下丘脑细胞培养中GnRH神经元的免疫细胞化学研究

采用免疫细胞化学方法，对分离培养１、３、５、７ｄ新生大鼠下丘脑促性腺激素释放激素（ＧｎＲＨ）免疫反应性神经元进行了研究。结果发现，在培养的１ｄ即可检出ＧｎＲｈ神经元，ＧｎＲＨ神经元占培养中的神经元的１２．１％￣１４．８％。ＧｎＲＨ神经元多为双极，有些突起末端可见生长锥。还发现ＧｎＲＨ神经元之间及其与其他神经元之间，存在有多种形式的接触。这些资料提示，培养中的ＧｎＲＨ神经元，保留了在体ＧｎＲＨ神经元

IMMUNOCYTOCHEMICAL STUDY OF GONADOTROPIN-RELEASING HORMONE (GnRH) NEURONS IN DISSOCIATED CULTURES OF NEWBORN RAT HYPOTHALAMUS

Gonadotropin-Releasing Hormone (GnRH) immunoreactive neurons in dissociated cell culture from newborn rat hypothalamus were investigated on days 1,3,5,and 7 in vitro by means of the im-munocytochemical method. The results showed that GnRH was expressed in the first day of culture. GnRH neurons accounted for 12.1-14. 8% of the total neurons in culture,and they were mainly bipolar in type. There were growth cones on the end of GnRH processes. Various patterns of intercellular contacts between GnRH neurons and between GnRH and other neurons were also observed. These findings indicate that the cultured GnRH neurons exhibit the morphological and functional characteristics of the GnRH neurons in vivo,and serve as morphological evidence for pulsatile secretion of GnRH and its regulation.