| 骨髓基质细胞对人多发性骨髓瘤细胞系自分泌白细胞介素6诱导作用的研究 |
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| 引用本文： | 王江方,张学光,顾宗江,冯树一,茅子均. 骨髓基质细胞对人多发性骨髓瘤细胞系自分泌白细胞介素6诱导作用的研究[J]. 中华血液学杂志, 1994, 0(11) |
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| 作者姓名： | 王江方 张学光 顾宗江 冯树一 茅子均 |
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| 作者单位： | 苏州医学院免疫研究室 |
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| 基金项目： | 国家教委优秀青年教师基金 |
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| 摘 要： | 研究了小鼠骨髓基质细胞对诱导人白细胞介素６（ＩＬ－６）依赖性多发性骨髓瘤（ＭＭ）细胞自分泌ＩＬ－６的作用。结果表明：人ＭＭ细胞系ＸＧ－１培养上清（ＳＮ＿１）没有可检测水平ＩＬ－６活性；小鼠骨髓基质细胞系ＭＦ－１培养上清（ＳＮ＿２）仅有低水平ＩＬ－６活性（１００Ｕ／ｍｌ）；ＸＧ－１细胞和ＭＦ－Ｌ细胞／上清共同培养上清（ＳＮ＿３，ＳＮ＿４）均含极高的ＩＬ－６活性（１０５０Ｕ／ｍｌ），并可被ＩＬ－６单抗ＢＥ－８特异性中和，而且不同浓度的ＳＮ＿３，ＳＮ＿４可以促进其他依赖外源性ＩＬ－６的人ＭＭ细胞系（ＸＧ－２，４，６）生长。鉴此证实了小鼠骨髓微环境基质细胞可以诱导ＭＭ细胞自分泌ＩＬ－６，后者可促进ＭＭ细胞生长。
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| 关 键 词： | 多发性骨髓瘤，白细胞介素6，基质细胞，多发性骨髓瘤细胞系，自分泌 |
INDUCTION OF AUTOCRINE IL-6 IN HuMAN MULTIPLEMYELOMA CELL LINE BY MURINE STROMAL CELLS |
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| Wang Jiangfang, Zhong Xueguang, Gu Zcogjiang,et al. INDUCTION OF AUTOCRINE IL-6 IN HuMAN MULTIPLEMYELOMA CELL LINE BY MURINE STROMAL CELLS[J]. Chinese Journal of Hematology, 1994, 0(11) |
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| Authors: | Wang Jiangfang Zhong Xueguang Gu Zcogjiang et al |
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| Abstract: | he roles of the murine stromal cells in inducing anautocrine IL-6 loop in human IL-6-dependent myelomacell line (XG-1) were studied. The results showed thatno IL-6 activity in the cultured supernatant of XG-1 cells(SN_1) and very low IL-6 activity in the cultured super-natant of murine stromal cell line MF-L(SN_2) were de-tected, very high level of IL-6 in the coculturedsuprnatant (SN_3 , SN_4) of MF-L cells/supernatant andXG-1 cells was found. Meanwhile, the activity of IL-6from SN_3/SN_4, which can stimulate the growth of otherIL-6-dependent multiple myeloma (MM) cell lines (XG-2 ,4 , 6) , can be totally inhibited by the specific mono-clonal antibody to IL-6 (BE-8).The study indicated that the stromal cells from murinebone marrow could rapidly induce an autocrine IL-6 loopin human IL-6-dependent MM cells and this loop pro-mote the tumor cells'growth without any exogenous IL-6. |
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| Keywords: | Multiple myeloma interleukin 6Stromal cell Muftiple myeloma cellline Autocrine |
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