柯萨奇B4病毒诱导的实验性糖尿病小鼠血清一氧化氮浓度的变化

目的:观察CB₄V诱导的实验性糖尿病小鼠血清NO浓度的变化以及在不同时期产生的IL-1水平。方法:建立CB₄V诱导的实验性糖尿病小鼠模型,分别在病毒感染后72h、1周、3周、6周、8周,用硝酸还原酶法测定血清NO^-₂/NO^-₃含量,同时测定经LPS诱导的巨噬细胞产生的IL-1水平。结果:(1)血清NO^-₂/NO^-₃浓度变化:对照组各时期血清NO^-₂/NO^-₃浓度无显著差异,模型组小鼠血清NO^-₂/NO^-₃浓度在CB₄V感染72h后显著增高(P＜0.01),在感染后1周达到高峰,6-8周时接近正常水平。(2)IL-1水平的测定:在CB₄V感染后72h,IL-1水平明显高于对照组(P＜0.01),1周达到高峰(P＜0.01),6周接近正常水平。在各时期NO^-₂/NO^-₃与IL-1均呈高度正相关。结论:NO可能参与CB₄V诱导的IDDM发生发展过程。

Changes of serum nitric oxide in CB4V-induced insulin-dependent diabetic mice

AIM: To observe the changes of serum nitric oxide and the production level of IL-1 in different period of coxsackievirus B4 (CB₄V)-induced insulin-dependent diabetic mice.METHODS: The insulin-dependent diabetes mellitus (IDDM) animal model induced by CB₄V infection was established. Serum nitric oxide level was estimated by nitrate reductase method after infection 72 h,1 week, 3 weeks,6 weeks,8 weeks, respectively. At the same time, level of IL-1 produced by peritoneal Mф was measued.RESULTS: (1) Changes of serum nitric oxide: serum nitric oxide level in control group remained normal level. The serum nitric oxide level in diabetic group increased significantly at 72 h after infection(P＜0.01), and reached top at 1 weeks after infection, then decreased to normal level in 6 and 8 weeks (P＞0.05). (2) IL-1 activities: IL-1 activities were increased obviously from 72 h to 3 weeks after virus infection, but decreased to normal level after 6 weeks.CONCLUSION: Nitric oxide may be one of the important factors in the development of CB₄V-induced IDDM.