Lipid peroxidation and scavenging enzyme activity in streptozotocin-induced diabetes |
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| Authors: | M Kinalski A Śledziewski B Telejko W Zarzycki I Kinalska |
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| Institution: | (1) Department of Pathophysiology of Pregnancy, Medical Academy of Białystok, M. Curie-Skłodowska Str. 24A, 15-273 Białystok, Poland, PL;(2) Department of Fetal and Neonatal Development, National Research Institute of Mother and Child, 15-273 Białystok M. Curie-Skłodowska 24A, Poland, PL;(3) Department of Endocrinology, Medical Academy of Białystok, M. Curie-Skłodowska 24A, 15-273 Białystok, Poland, PL |
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| Abstract: | The aim of this study was to evaluate lipid peroxidation and scavenging enzyme activity in streptozotocin-induced diabetes,
and then to establish whether moderate doses of nonenzymatic antioxidant vitamin E play a role in the antioxidant defence
system in diabetic pregnant rats and their offspring. The study group consisted of 30 normal female Wistar rats, which were
given a single dose of streptozotocin (40 mg/kg) and were mated 7 days later. Subsequently, the diabetic animals were divided
into two matched groups: the first supplemented with vitamin E (30 mg/100 g chow), and the other fed with a standard diet
lacking vitamine E. Controls consisted of 15 pregnant rats. On the first day after delivery, the rats were decapitated and
homogenates of maternal liver and uterus as well as neonatal lungs and liver were prepared. Then the following parameters
were measured: malondialdehyde (MDA) concentrations in the homogenates and blood serum, glutathione (GSH) levels, the activity
of CuZn-superoxide dismutase (SOD) and glutathione peroxidase (GPx), and glycaemia. The neonates of diabetic rats were smaller
than the healthy ones and serum glucose concentration was markedly higher in the diabetic animals. MDA levels were significantly
increased, whereas GSH, SOD and GPx were markedly diminished in the diabetic adult rats and their offspring in comparison
to the control grouop. In the animals supplemented with α-tocopherol, MDA concentrations were significantly lower, GSH content
and SOD activities were markedly elevated most tissues studied, whereas GPx remained unchanged. We conclude that, by monitoring
the activity of selected scavenging enzymes, information on ongoing biological oxidative stress and thereby on the fetus/neonate
status may be obtained. Our results suggest that diabetic pregnant rats and their neonates are exposed to an increased oxidative
stress and that vitamin E supplementation may reduce its detrimental effects.
Received: 20 January 2000 / Accepted in revised form: 23 February 2001 |
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| Keywords: | Pregnancy Hyperglycaemia Oxidative stress Lipid peroxidation Vitamin E |
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