β3—肾上腺素能受体基因与肥胖关系

目的 探讨β3－肾上腺素能受体（β3－AR）基因Trp64Arg突变与肥胖的关系。方法 采用聚合酶链反应－限制性酶切片段长度多态性技术检测β3－AR基因型；同时检查体质指数（BMI）等指标，观察在147例肥胖患和106例健康对照中，β3－AR基因型与Arg64等位基因的频率分布以及不同体质指数的人群中，其突变基因的分布情况。结果 肥胖组Arg64等位基因的频率（20．3％）显高于对照组（10．2％）（χ^2=10.14,P=0.001)，突变基因型Trp64Arg、Arg64Arg的分布频率38．7％和0．9％）也高于对照组（19．0％和0．07％）。在女性人群中，肥胖Trp64Arg基因型及Arg64等位基因频率（40．6％和20．3％）显高于非肥胖（16．7％和9．5％），且差异有显性（P＜0．01）。结论 β3－肾上腺素能受体基因Trp64Arg突变可能与本脂增多有关。

Study on association of the Beta 3-adrenergic receptor gene wit h obesity

Objective　To investigate the association betwee n the β3-adrenergic receptor gene (β3-AR) and obesity. Methods　147 patients with obesity and 106 controls were selected. Genotype of the β 3-AR was determined by polymerase chain reaction (PCR) and restriction fragme nt length polymorphism methods (RFLP). Body mass index (BMI) was detected. Results　The frequency of Arg64 allele of β3-AR gene in the obesit y g roup (20.3%) was significantly higher than those in the control group (10.2%) ( P=0.001) . The distribution of Trp64Arg and Arg64Arg genotype in the patient group (38.7% and 0.9%) were both higher than that in the control group (19.0% and 0.7%). In the female subj ects, the Trp64Arg mutation and frequency of Arg64 allele of the β3-AR gene in the obesity group(40.6% and 20.3%) were also higher than those in control (16 . 7% and 9.5%)(P<0.01). Conclusions　These results suggest th at the Trp64Arg mutation of the β3-adrenergic-receptor gene may be associat ed with increased body fat.