| 石柱黄连指纹图谱研究 |
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| 引用本文： | 黄小平,李隆云,瞿显有,崔广林.石柱黄连指纹图谱研究[J].中药材,2006,29(7):666-669. |
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| 作者姓名： | 黄小平 李隆云 瞿显有 崔广林 |
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| 作者单位： | 重庆市中药研究院,重庆,400065 |
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| 摘 要： | 目的：建立石柱道地药材黄连的HPLC指纹图谱，为科学评价与有效控制黄连质量提供新方法。方法：RP-HPLC法测定石柱GAP基地10批黄连样品。色谱条件：迪马Diamonsil C18色谱柱，乙腈-0．05mol／L磷酸二氢钾（磷酸调pH值3）为流动相进行梯度洗脱，检测波长270nm，流速0．8ml／min，柱温25％。结果：10批黄连样品得到的指纹图谱有16个共有峰，通过与对照品的保留时间及紫外光谱比较，11，12，13，14，15号峰分别为伪小檗碱、药根碱、黄连碱、巴马汀和小檗碱。结论：建立的HPLC指纹图谱分析法能较好反映黄连中的主要生物碱成分，可用于黄连的质量控制。
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| 关 键 词： | 黄连 高效液相色谱法 指纹图谱 |
| 文章编号： | 1001-4454(2006)07-0666-04 |
| 收稿时间： | 2006-02-08 |
| 修稿时间： | 2006年2月8日 |
Fingerprints of Rhizoma Coptidis from Shizhu by HPLC |
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| HUANG Xiao-ping,LI Long-yun,QU Xian-you,CUI Guang-lin.Fingerprints of Rhizoma Coptidis from Shizhu by HPLC[J].Jorunal of Chinese Medicinal Materials,2006,29(7):666-669. |
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| Authors: | HUANG Xiao-ping LI Long-yun QU Xian-you CUI Guang-lin |
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| Institution: | Chongqing Academy of Chinese Materia Mediea, Chongqing 400065, China |
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| Abstract: | OBJECTIVE: To establish a sensitive and specific HPLC method for the quality control of Rhizoma Coptidis collected from shizhu in chongqing. METHODS: The HPLC fingerprints of Rhizoma Coptidis from shizhu were obtained from Waters instrument. The methods was performed on a Diamonsil C18 column gradient eluted with acetonitrile-0.05 mol/L KH2PO4 (pH3 with H3PO4) at the flow rate of 0.8 m/min. The temperature of column was 25 degrees C and the UV detection wavelength was 270nm. RESULTS: The HPLC fingerprint of Rhizoma Coptis, showing 16 characteristic peaks, was established from 10 lots of Rhizoma Coptis. By comparision of the retention time and the on-line UV spectra of chemical standards, peak 11, 12, 13, 14 and 15 were identified as Epiberberine, Jatrorrhizine, Coptisine, Palmatine and Berberine. CONCLUSION: The HPLC fingerprint of Rhizoma Coptidis with high specificity can be used to control its quality. |
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| Keywords: | Rhizoma Coptidis HPLC Fingerprint |
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