| MPTP对小鼠纹状体多巴胺含量的影响 |
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| 引用本文： | 盛锦根,徐德隆,余慧贞,唐琴敏,徐修蓉. MPTP对小鼠纹状体多巴胺含量的影响[J]. 上海交通大学学报(医学版), 1987, 0(2) |
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| 作者姓名： | 盛锦根 徐德隆 余慧贞 唐琴敏 徐修蓉 |
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| 作者单位： | 上海第二医科大学附属瑞金医院神经科(盛锦根，徐德隆，余慧贞)，中国科学院上海药物研究所(唐琴敏)，中国科学院上海药物研究所(徐修蓉) |
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| 摘 要： | 报道1—甲基—4—苯基—1,2,3,6—四氢吡啶(MPTP)给C_(57)-BL黑色小鼠腹腔注射后,应用高效液相色谱—电化学检测法,测定纹状体多巴胺(DA)、去甲肾上腺素(NA)、5—羟色胺(5-HT)及其代谢产物3,4—二羟基苯乙酸(DOPAC)、高香草酸(HVA),5—羟基吲哚乙酸(5-HIAA)的含量。结果与对照组相比,用药组小鼠纹状体DA、5-HT及其代谢产物显著降低,其中DA减少94%(P<0.001),DOPAC减少61%(P<0.05),HVA减少65%(P<0.001)。实验结果表明用MPTP给予小鼠所产生的动物模型,对研究帕金森病是一种较为有用的方法。
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| 关 键 词： | MPTP 纹状体 高效液相色谱—电化学检测法 多巴胺 |
Changes of Striatal Dopamine Induced by MPTP in Mice |
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| Sheng Jingen,et al. Changes of Striatal Dopamine Induced by MPTP in Mice[J]. Journal of Shanghai Jiaotong University:Medical Science, 1987, 0(2) |
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| Authors: | Sheng Jingen et al |
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| Affiliation: | Sheng Jingen,et al Department of Neurology. Ruijin Hospital,Shanghai Second Medical Unviersity,Shanghai |
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| Abstract: | 1-methy1-4-pheny1-1,2,3, 6-tetrahydropyridine (MPTP) was injected to C57-BL black mice, 36mg/kg, for 7 days, 12 days after the last MPTP injection, the mice were decapitated and the striatum was dissected out. Contents of dopamine (DA), 3, 4-dihydroxy-phenylacetic acid (DOPAC), homovanillic acid (HVA), serotonin(5-HT), 5-hydroxyindoleacetic acid(5-HIAA), norepinephrine (NA) were measured in striatal homogenates by high perfomance liquuid chromatography with eletrochemical detection. In the mice treated with MPTP, the average levels as compared with the control were reduced by 94% for DA (P<0.001), 62% for DOPAC(P<0.05), 65% for HVA (P<0.001). Hence, MPTP-treated mice mayprove to be a useful model to study Parkinson's disease. |
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| Keywords: | MPTP striatum high performance liquid chromatography electrochemical detection dopamine |
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