抗凝剂EDTA及枸橼酸钠导致血小板假性减少现象的分析

目的:分析抗凝剂EDTA及枸橼酸钠所致血小板聚集对血小板计数结果的影响及观察仪器法和手工计数法两种方法检测EDTA及枸橼酸钠所致血小板聚集标本的不同结果。方法:血小板仅对EDTA发生聚集反应的标本为一组,血小板对EDTA及枸橼酸钠均发生聚集反应的标本为二组。用Sysm ex-2100血液分析仪检测上述两组EDTA抗凝血和枸橼酸钠抗凝血,同时采指血手工计数血小板和抗凝血涂片显微镜镜检。结果:一组中EDTA抗凝血血小板数值明显低于枸橼酸钠抗凝血,枸橼酸钠抗凝血血小板数值与手工计数法结果相接近。二组中EDTA抗凝血血小板数值与枸橼酸钠抗凝血结果相近,均明显低于血小板手工法结果。结论:除EDTA引起的血小板聚集可导致血小板数值假性减低外,极个别情况下,血小板也对枸橼酸钠发生聚集反应引起血小板假性减低。对于首次检测血小板数值减低的标本均应手工计数血小板或涂片镜检,以确认是否存在抗凝剂所致血小板聚集现象。

Analysis on phenomenon of anticoagulants EDTA and citrate sodium led to false platelet reducing

Objective:To analyze platelet aggregation induced by anticoagulant EDTA and sodium citrate and observe the different outcomes between equipment and manual platelet counting on EDTA and sodium citrate induced platelet aggregation samples.Methods:Samples which platelet aggregation can be induced by EDTA were named group one.Samples which platelet aggregation can be induced by both EDTA and sodium citrate were named group two.Sysmex-2100 hematology analyzer was used on detecting group one and two.Manual platelet counting and smear microscopy examination on periphery blood was also performed.Results:In group one,Platelets counts in anti-clotting blood by EDTA was significantly lower than anti-clotting blood by anticoagulant sodium citrate.Platelets counts in anti-clotting blood by sodium citrate anticoagulation was similar to manual platelet counting.In group two,Platelets counts in anti-clotting blood by EDTA was similar to anti-clotting blood by anticoagulant sodium citrate.The results was significantly lower than manual platelet counting.Conclusion:Except EDTA induced platelet aggregation can lead to false numerical reduction,in a few circumstances,sodium citrate induced platelet aggregation can also caused false platelet reduce.Manual platelet counting or smear microscopy examination should be done on blood samples which platelet reduction was discovered for the first time to confirm the phenomenon of platelet aggregation induced false platelet reducing.