硫酸阿米卡星对EDTA依赖性假性血小板减少症血小板聚集的解离作用

目的：探讨硫酸阿米卡星对乙二胺四乙酸(EDTA)依赖性假性血小板减少症（EDTA-PTCP）患者血标本中血小板聚集的解离作用，为这类标本血小板的准确计数提供有效方法。方法：在1例少见的EDTA-PTCP患者EDTA-K2抗凝管中预先加入或不加硫酸阿米卡星后采集静脉血，分别于采血后不同时间用全自动血细胞分析仪检测血小板数和其他血细胞参数；留取EDTA-K2抗凝血后不同时间加入阿米卡星，观察其对血小板聚集的解离作用，并检查血涂片中血小板形态改变。结果：未加阿米卡星的EDTA-PTCP标本，随采血后标本放置时间（0.5~4.0 h）的延长，血小板数从117×109 L-1逐渐降至41×10⁹ L^-1。在EDTA-K2抗凝管中预先加入阿米卡星的血标本，血小板计数在正常参考值范围内，且标本室温放置4.0 h内血小板计数值相对稳定。采集的血标本放置30 min以内加入阿米卡星，血小板计数（186×109 -1~191×10⁹ L^-1）在正常参考值范围内，血涂片镜检可见聚集的血小板明显解离，而超过30 min后加入硫酸阿米卡星，随标本放置时间（40 min~4 h）的延长，血小板计数从放置40 min时的168×109 L^-1逐渐降至放置4 h时的42×109 L^-1，镜检血涂片中血小板逐渐明显聚集。结论：留取血标本前或留取标本后30 min内加入一定量硫酸阿米卡星，全自动血细胞分析仪检测EDTA-PTCP患者血小板计数是准确的，且其他血细胞参数的测定未受影响。

Dissociative effect of amikacin sulfate on platelet aggregation in EDTA-dependent pseudothrombocytopenia

Objective To investigate the dissociative effect of amikacin sulfate on platelet aggregation in patients with EDTA-dependent pseudothrombocytopenia (EDTA-PTCP),and develop an effective method for accurate platelet counting in this kind of samples.Methods Amikacin sulfate was added or not to the EDTA-K2 anticoagulated tube before collecting venous blood in an infrequent patient with EDTA-PTCP,and the platelet counts and other blood cell parameters at different time after blood withdrawal were examined by aut...