CD44V6在大肠癌转移中作用机理的研究

目的研究ＣＤ４４Ｖ６在大肠癌转移中的作用机理。方法应用羊膜代基底膜模型并将大肠癌肿瘤细胞与羊膜及血管内皮共同培养,研究ＣＤ４４Ｖ６对大肠癌细胞粘附基底膜与血管内皮的影响。采用共聚焦图像三维重构分析技术研究ＣＤ４４Ｖ６对大肠癌细胞骨架的影响。使用激光漂白后荧光恢复技术研究ＣＤ４４Ｖ６对大肠癌细胞缝隙连接细胞间通讯的影响。结果ＣＤ４４Ｖ６单抗能抑制大肠癌细胞对羊膜及血管内皮的粘附和浸润能力,影响大肠癌细胞骨架蛋白的构型和分布。但对大肠癌细胞缝隙连接细胞间通讯无明显影响。结论ＣＤ４４Ｖ６变异体能促进大肠癌细胞与血管内皮细胞及基底膜的粘附和浸润,并影响细胞骨架蛋白的构型和分布,增强肿瘤细胞的运动能力,促进肿瘤转移的发生

Mechanism of CD44V6 in human colorectal carcinoma metastasis

Objective To study the mechanism of CD44V6 in human colorectal carcinoma (HCC) matastasis. Methods The affects of CD44V6 on HCC cell adhering to base membrane and vascular endothelium were investigated by human amniotic membrane invasion model and co culturing HCC cell with vascular endothelium .The influence of CD44V6 variants on cytoskeleton and gap junctional intercellular communication (GJIC) was studied by immunofluorescent confocal 3 dimensional reconstruction and fluorescence recovery after photobleaching technique. Results CD44V6 antibody can decrease the ability of HCC cells to adhere to amniotic membrane and vascular endothelium. It also can affect the distribution, polymerization and depolymerization of actin of HCC cells, but does not significantly affect GJIC of HCC cells. Conclusions CD44V6 may play an important role in promoting HCC cells to adhere to vascular endothelium and base membranes and may affect the distribution, polymerization and depolymerization of actin in HCC cells and facilitate HCC cell metastasis.