乳腺癌合并肥胖症患者血浆瘦素水平与乳腺癌组织中瘦素受体mRNA的表达及临床病理的关系

检测乳腺癌合并肥胖症患者乳腺癌组织中瘦素受体(leptin receptors)mRNA的表达情况和血浆瘦素水平,并探讨二者之间及其与临床病理之间的关系.对124例单纯肥胖者,或乳腺癌合并肥胖者,或乳腺良性病变合并肥胖者同时采集病史、进行体格检查并留取血浆,测定其瘦素的水平.采用半定量逆转录-聚合酶链反应(RT-PCR)检测瘦素受体mRNA的表达.结果显示,乳腺癌合并肥胖组血浆瘦素水平显著高于乳腺良性病变合并肥胖组及单纯肥胖组(P＜0.05).瘦素长型受体[Lep-R(L)]mRNA及瘦素短型受体[Lep-R(S)]mRNA在乳腺癌合并肥胖组中的表达水平均明显高于乳腺良性病变合并肥胖组(P＜0.05).血浆瘦素水平与乳腺组织中Lep-R(L)mRNA及Lep-R(S)mRNA水平呈显著正相关.血浆瘦素和瘦素受体mRNA的表达与腋窝淋巴结转移、绝经、TNM分期及病理类型均无显著相关性(P＞0.05).结论:瘦素可能参与了乳腺癌的发生.

Relationship Between Expression of Leptin Receptors mRNA in Breast Tissue, Plasma Leptin Level in Breast Cancer Patients with Obesity and Clinical Pathologic Data

In order to investigate the expression of leptin receptors mRNA in breast tissue and plasma leptin levels in breast cancer patients with obesity and their relationship with clinical pathologic datas,124 subjects who were either obesity or had suffered from breast benign disease with obesity,or breast cancer with obesity were entered into this study.The levels of plasma leptin in all subjects were determined and leptin receptors mRNA expression levels were measured by RT-PCR in breast tissue of breast cancer patients with obesity and breast benign disease with obesity.The results showed that plasma leptin levels in breast cancer patients with obesity were significantly higher than those in breast benign disease with obesity and obesity patients alone(P<0.05).The expression of the leptin receptor long form mRNA and the leptin receptor short form mRNA in breast tissue of breast cancer patients with obesity were significantly higher than that in breast tissue of breast benign disease patients with obesity(P<0.05).The plasma leptin level had remarkable positive correlation with the expressions of the Lep-R(L) mRNA and the Lep-R(S) mRNA.The plasma leptin level and leptin receptors mRNA expression levels in patients were not correlated with the axillary node metastasis,menopause,the TNM stage or pathological type.Therefore,leptin may have a promoting effect on the carcinogenesis of breast cancer.