| 三种非水溶性供试品溶剂对抑菌试验效果的影响 |
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| 引用本文： | 胡君茹,姜 华,李喜香.三种非水溶性供试品溶剂对抑菌试验效果的影响[J].甘肃中医,2013(11):30-31. |
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| 作者姓名： | 胡君茹 姜 华 李喜香 |
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| 作者单位： | [1]甘肃省中医药研究院,甘肃兰州730050 [2]甘肃省中医院,甘肃兰州730050 |
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| 摘 要： | 目的：考察吐温-80、二甲基亚砜（DMSO）、1，2-丙二醇3种非水溶性物质溶剂，在抑菌试验中溶解非水溶性供试品时对抑茵效果的影响。方法：通过体外抑菌试验，用琼脂扩散法测定3种溶剂的抑菌作用。结果：DMSO与水混合的不同浓度对金黄色葡萄球菌和大肠杆菌均无抑菌作用，在抑菌试验中可用于溶解非水溶性供试品，而吐温-80和1，2-丙二醇对抑茵效果有干扰。结论：在考察非水溶性供试品的抑菌活性时，DMSO对抑菌结果无干扰，可作为适宜的溶剂溶解非水溶性供试品。
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| 关 键 词： | 吐温-80 DMSO 1 2-丙二醇 非水溶性溶剂 抑菌作用 |
Study on Three Non-water-soluble Solvents influencing of Inhibitory Activity in Antibacterial Test |
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| Institution: | HU Junru, JIANG Hua, LI Xixian(1 Gansu Provincial Academy of Chinese Medicine, Lanzhou 730050, China; 2 Gansu Provincial Hospital of Traditional Chinese Medicine) |
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| Abstract: | Objective： To survey the effects oftween-80, dimethyl sulfoxide （DMSO） and 1, 2- propanediol on inhibitory activity in antibacterial test. Method： Inhibitory activity of all the solvents was detected by agar disc diffu- sion method for antibacterial test in vitro. Result： Different concentrations of DMSO mixed liquid showed no inhibi- tion to Staphylococcus aureus and Escherichia coli while Tween 80 and 1, 2-propanediol demonstrated interference to antibacterial results. Conclusion： In the investigation of inhibitory activity of non-water-soluble solvents, DMSO has no interference to antibacterial results and is suitable for dissolving non-water-soluble solvents in antibacterial test. |
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| Keywords: | ween-80 DMSO 1 2- propanediol non-water-soluble solvents inhibitory activity |
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