| HPLC-UV-ELSD联用检测熊香胶囊中牛磺熊去氧胆酸的含量 |
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| 引用本文： | 柏冬,彭娟,闫寒,范斌.HPLC-UV-ELSD联用检测熊香胶囊中牛磺熊去氧胆酸的含量[J].辽宁中医杂志,2010(6):1108-1109. |
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| 作者姓名： | 柏冬 彭娟 闫寒 范斌 |
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| 作者单位： | [1]北京中医药大学,北京100029 [2]中国中医科学院医学实验中心,北京100700 |
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| 摘 要： | 目的:HPLC-UV-ELSD联用检测熊香胶囊中牛磺熊去氧胆酸的含量;方法:采用Kromasil C18色谱柱(5μm,4.6mm×250mm),以甲醇-水(0.1%甲酸)(60∶40)为流动相;紫外检测波长为210nm;ELSD漂移管温度105℃,雾化气体(空气)流速2.7L·min-1;结果:牛磺熊去氧胆酸在1.44～5.76μg线形关系良好,紫外检测方式回收率为97%(n=5),RSD2.3%,UV检测方式回收率为101%(n=5),RSD1.5%;结论:与HPLC/UV相比,HPLC/ELSD测定熊香胶囊中牛磺熊去氧胆酸成分的含量结果更准确。
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| 关 键 词： | 熊香胶囊 牛磺熊去氧胆酸 HPLC UV ELSD 含量测定 |
Determination of TUDCA in Xiongxiang Capsule by HPLC - UV - ELSD |
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| BAI Dong,PENG Juan,YAN Han,FAN Bin.Determination of TUDCA in Xiongxiang Capsule by HPLC - UV - ELSD[J].Liaoning Journal of Traditional Chinese Medicine,2010(6):1108-1109. |
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| Authors: | BAI Dong PENG Juan YAN Han FAN Bin |
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| Institution: | 1.Beijing University of Chinese medicine,Beijing 100029,China;2.Medical Experiment Center,China Academic of Chinese Medicine Sciences,Beijing 100700,China) |
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| Abstract: | Objective： To establish a method for the determination of TUDCA in Xiongxiang capsule by HPLC-UV-ELSD.Methods： A HPLC methods detected by UV and ELSD has been developed： Kromasil C18 column （ 250mm × 4.6mm,5μm） with mobile phase of methane and 0.1% formic acid;the detection wavelength was 210 nm;The temperature of the drift tube was set at 105℃,the flow rate of gas at 2.7L·min -1.Results： TUDCA in Xiongxiang Capsule could be determined by HPLC-UV and HPLC-ELSD.The recovery was 97% （ n = 5,RSD = 2.3% ） for HPLC/UV,101% （ n = 5,RSD = 1.5% ） for HPLC/ELSD,respectively.Conclusion： Comparing with HPLC/UV,The HPLC/ELSD method was more accurate and specific to determine the content of TUDCA in Xiongxiang capsule. |
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| Keywords: | Xiongxiang Capsule TUDCA HPLC UV ELSD content determination |
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