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| 聚乙二醇修饰的蓖麻毒蛋白A链的特性研究 | |
| 引用本文： | 黎维勇,方凯,郭文韬,戴垚,谌辉,陈华庭. 聚乙二醇修饰的蓖麻毒蛋白A链的特性研究[J]. 中国医院药学杂志, 2006, 26(8): 915-918 |
| 作者姓名： | 黎维勇 方凯 郭文韬 戴垚 谌辉 陈华庭 |
| 作者单位： | 1. 华中科技大学同济医学院附属协和医院,湖北,武汉,430022 2. 华中科技大学生命科学与技术学院,湖北,武汉,430074 3. 中国医学科学院中国协和医科大学医药生物技术研究所,北京,100050 |
| 摘 要： | 目的：研究两种不同相对分子质量（Mr）的单甲氧聚乙二醇（Monomethoxy polyethylene glycol，mPEG）与蓖麻毒蛋白A链（ricin A-chain，RTA）偶联后三者的免疫原性和毒性的不同。方法：从天然蓖麻籽中提取纯化的RTA，并分别与相对分子质量为5KD和10KD的mPEG反应偶联，得到两种不同的RTA-mPEG偶联物。用蛋白质印迹方法比较RTA和不同的RTA-mPEG偶联物之间的免疫原性的变化，此外通过用噻唑蓝比色法（MTT）对RTA及不同偶联物的体外细胞毒性进行评价，并通过测定半数致死量（LD50）的方法比较它们的体内毒性。结果：经聚乙二醇修饰后的RTA，其毒性有明显的下降。此外，对其免疫原性的研究表明，RTA的PEG化也能降低RTA的免疫原性。结论：聚乙二醇能遮蔽RTA，使其免疫原性和毒性有很大的降低。且下降的程度同与RTA偶联的聚乙二醇的相对分子量成正相关。 |
| 关 键 词： | 蓖麻毒蛋白A链 聚乙二醇 免疫原性 毒性 |
| 文章编号： | 1001-5213(2006)08-0915-04 |
| 收稿时间： | 2005-12-21 |
| 修稿时间： | 2005-12-21 |
Study on the properties of Ricin A-chain modified by polyethylene glycol |
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| LI Wei-yong,FANG Kai,GUO Wen-tao,DAI Yao,CHEN Hui,CHEN Hua-ting. Study on the properties of Ricin A-chain modified by polyethylene glycol[J]. Chinese Journal of Hospital Pharmacy, 2006, 26(8): 915-918 | |
| Authors: | LI Wei-yong FANG Kai GUO Wen-tao DAI Yao CHEN Hui CHEN Hua-ting |
| Affiliation: | 1. Union Hospital Affliated to Tongji Medical College, Huazhong University of Science and Technology, Hubei Wuhan 430022, China; 2. School of Life Science and Technology, Huazhon4g University of Science and Technology, Hubei Wuhan 430074, China; 3. Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences Peking Union Medical College, Beijing 100050, China |
| Abstract: | OBJECTIVE To study the difference in immunogenicity and toxicity of ricin A-chain(RTA) and RTA-mPEGs.METHODS RTA and PEG-RTA conjugates modified by polyethylene glycol (PEG) with different MW ( 5 000 and 10 000 ) were prepared. The immunogenicity of RTA and RTA-mPEG was compared by Western blot method. The in vivo and in vitro toxicity was compared by the determination of LD_ 50 and MTT method respectively. RESULTS The immunogencity and toxicity of RTA modified by mPEG were reduced remarkably. CONCLUSION Immunogenicity and toxicity of RTA-mPEG decrease significantly compared with free RA probably as PEG can cover RTA. The decreasing txtent is proportional to molecular weight of the PEG. |
| Keywords: | Ricin A-chain polyethylene glycol immunogenicity toxicity |
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