| 脑胶质瘤浸润淋巴细胞的分离、培养与表型及体外抗肿瘤活性测定 |
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| 引用本文： | 康德智,张国安,刘小朋,陈锦峰,陈紫榕. 脑胶质瘤浸润淋巴细胞的分离、培养与表型及体外抗肿瘤活性测定[J]. 福建医科大学学报, 1994, 0(2) |
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| 作者姓名： | 康德智 张国安 刘小朋 陈锦峰 陈紫榕 |
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| 作者单位： | 福建医学院附属第一医院神经外科，空军福州医院临床免疫研究室 |
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| 摘 要： | 经重组白细胞介素２（ｒＩＬ－２）和胸腺因子Ｄ（ＴＦＤ）培养前后的人脑胶质瘤浸润淋巴细胞（ＧＩＬ）的表型及抗自体瘤细胞活性变化。（１）ＧＩＬ可被分离，培养后可增值；（２）新鲜ＧＩＬ主要为Ｔ淋巴细胞，其中ＣＤ＋８细胞多于ＣＤ４细胞，随培养时间延长呈相反变化；（３）新鲜ＧＩＬ活性很弱，培养后很强；（４）ＴＦＤ可能只有增强ｒＩＬ－２的激活作用。提示ＧＩＬ可望作为理想的效应细胞用于脑胶质瘤的治疗。
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| 关 键 词： | 脑浸润淋巴细胞，肿瘤，白细胞介素2，胸腺因子D，培养，免疫细胞毒性 |
Isolation,Culture,Phenotype and in Vitro Antitumor Cytolytic Activity or Glioma-infiltrating Lymphocytes of Brain |
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| Kang Dezhi,Zhang Guoan,Liu Xiaopeng,et al. Isolation,Culture,Phenotype and in Vitro Antitumor Cytolytic Activity or Glioma-infiltrating Lymphocytes of Brain[J]. Journal of Fujian Medical University, 1994, 0(2) |
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| Authors: | Kang Dezhi Zhang Guoan Liu Xiaopeng et al |
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| Abstract: | We report the changes of phenotype and antitumor cytolytic activity of glioma-infiltrating lymphocytes(GIL)before and after recombinant interleukin-2(rIL-2)and thymic factor D(TFD).The results indicated that(a)GIL could be isolated from the human gliomas and proliferate after initiated in vitro;(b)most of fresh GIL were T lymphocytes,in which CD8 cells were more than CD4 cells, while the former became declining and the latter growing with time prolonged in culture;(c)killing activity of fresh GIL was weak,but that of initiat ed GIL was strong;(d)TFD could potentiate the initiated roles of rIL-2.Therefroe,it is reasonable to believe that GIL will be used as an ideal effector cells in the treatment of human brain gliomas. |
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| Keywords: | cerebral neoplasm tumor infiltrating lymphocyte interleukin-2 thymic factor D culture immunologic cytotoxicity |
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