黄芪多种成分抗人疱疹病毒的初步实验研究

目的：为了开发利用黄芪的抗病毒性质，我们进行了此项研究。方法：我们以阿普洛韦（ACV)为阳性对照，采用对病毒所致细胞病变的抑制及空斑减数实验，观察了黄芪总皂苷、总多糖、总黄酮抗HSV药效。结果：在Hep-2细胞系统中，ACV对HSV－1 HS－1株直接杀灭、感染阻断、增殖抑制的ED50为30．83ug/ml,16.04ug/ml,20.04ug/ml；对HSV－2333株的相应ED50为16.45ug/ml,18.62ug/ml,10.85ug/ml,黄芪总皂苷对HSV－1 HS－1株相应ED50为1．68ug/ml，1．72ug/ml，1．95ug/ml；对HSV－2333株相应ED50为1．73ug/ml，2．70ug/ml，2．74ug/ml，黄芪总多糖对HSV－1 HS－1株的相应ED50为4．03ug/ml，5．33ug/ml，4．90ug/ml；对HSV－2333株相应ED50为6．04ug/ml，5．43ug/ml，7．50ug/ml，黄芪总黄酮对HSV－1 HS－1株的相应ED50为4．95ug/ml，2．75ug/ml，3．49ug/ml；对HSV－2333株的相应ED50为3．56ug/ml，3．93ug/ml，6．27ug/ml。结论：黄芪对HSV－1的治疗指数为ACV的10．3，4．1，5．5倍；对HSV－2的治疗指数为ACV的3．7，2．7，1．0，1．6倍。总多糖对HSV－1的治疗指数为ACV的10．3，4．1，5．5倍；对HSV－2的治疗指数为ACV的3．7，2．7，3．3倍。总黄酮的治疗指数为ACV相近，值得开发利用。

The experimental research of antiherpes simplex virus effects of various components in the root of Astragalus membranaceus

OBJECTIVE:In order to understand and exploit the antiviral properties of Astragalus membranaceus. METHOD:ACV was used as positive control of antiherpes,through inhibitions of cellular pathological effects of herpes simplex virus type-2 and plaque reduction assays,the effects of antiherpes of total saponins ,total polysaccharieds,total flavonoids of Astragalus membranaceus were observed. RESULTS:In Hep-2 cell systems,the median effect doses(ED50) of ACV of direct virucidal activity,the inhibition effects on the infection and multiplication of HSV-1 HS-1 strain were 30.83ug/ml,16.04ug/ml and 20.04ug/ml, respectively the corresponding figures to HSV-2 333 strain were 16.45ug/ml,18.62ug/ml and 10.85ug/ml, respectively; the corresponding ED50s of total saponins to HSV-1 HS-1 strain were 1.68ug/ml,1.72ug/ml and 1.95ug/ml,respectively; those to HSV-2 333 strain were 1.73ug/ml,2.70ug/ml and 2.74ug/ml, respectively; the corresponding ED50s of total polysaccharides to HSV-1 HS-1 strain were 4.03ug/ml,5.33ug/ml and 4.90ug/ml, respectively; those to HSV-2 333 strain were 6.04ug/ml,5.43ug/ml and 7.50ug/ml, respectively.The corresponding ED50s of total flavonoids to HSV-1 HS-1 strain were 4.95ug/ml,2.75ug/ml and 3.49ug/ml, respectively; to HSV-2 333 strain were 3.56ug/ml,3.93ug/ml and 6.27ug/ml, respectively. CONCLUSION:The Treatment Indices(TI) of total saponins to HSV-1 HS-1 strain were 4.4,2.4 and 2.5times of ACVTIs,to HSV-2 333 strain were 2.3,1.0 and 1.6 times of ACVTIs.The TIs of total polysaccharides to HSV-1 HS-1 strain were 10.3,4.1 and 5.5 times of ACVTIs;to HSV-2 333 strain were 3.7,2.7 and 3.3 times of ACVTIs.The TIs of total flavonoids were close to ACVTIs.These components of Astragalus membranaceus should be worth exploitation.