丹酚酸B与大鼠血浆蛋白结合率的测定

建立测定丹酚酸B与大鼠血浆蛋白结合率的方法; 体外采用平衡透析法, 模拟丹酚酸B与血浆蛋白的结合过程, 体内采用超滤法, 并以高效液相色谱法进行测定。透析内液用甲醇沉淀蛋白, 透析外液过滤后直接测定。结果透析外液线性范围为0.5～20 μg·mL⁻¹, 透析内液的线性范围为2～200 μg·mL⁻¹, 提取回收率68.6%～81.9%, 日内日间精密度均小于8.5%, 丹酚酸B的体外血浆蛋白结合率为75.2%, 体内血浆蛋白结合率为92.1%, 丹酚酸B与大鼠血浆蛋白结合率较高。建立的方法灵敏度高, 重现性好, 操作简单, 能够满足分析要求。

Determination of the binding rate of rat plasma protein with salvianolic acid B

This paper is aimed to report the development of a method for the determination of the binding rate of plasma protein with salvianolic acid B.  In vitro, equilibrium dialysis method was used to imitate the binding process between salvianolic acid B and plasma protein, in vivo, ultrafiltration method was used and the binding rate with HPLC was determined.  Plasma samples were treated with methanol to precipitate the protein, and the buffer solution was directly determined after filtering.  The calibration curve of the buffer solution was linear in the range of 0.5 − 20 μg·mL⁻¹.  The calibration curve of the plasma was linear in the range of 2 − 200 μg·mL⁻¹.  The extract recovery was 68.6% − 81.9%.  RSDs of intra- and inter-day precisions were all less than 8.5%.  The binding rates of plasma protein with salvianolic acid B in vitro was 75.2% and in vivo was 92.1%.  This paper shows the high binding power of salvianolic acid B to plasma protein with high sensitivity, good   reproduction, simple management and fulfilling the requirement.