岩黄连生物碱类成分及其抗氧化活性研究

目的 研究岩黄连Corydalis saxicola的生物碱类成分及其抗氧化活性。方法 应用多种柱色谱法进行分离纯化，根据化合物的波谱分析进行结构鉴定，采用DPPH自由基清除法进行抗氧化活性研究。结果 从岩黄连全草70%乙醇提取物中共分离得到16个化合物，分别鉴定为卡维汀（1）、刺罂粟碱（2）、氢化小檗碱（3）、四氢巴马汀（4）、碎叶紫堇碱（5）、斯氏紫堇碱（6）、原阿片碱（7）、去氢碎叶紫堇碱（8）、脱氢异阿朴卡维汀（9）、小檗碱（10）、去氢分离木瓣树胺（11）、白屈菜红碱（12）、脱氢卡维汀（13）、黄堇碱（14）、异紫堇定碱（15）、深山黄堇碱（16）。体外抗氧化活性结果显示岩黄连生物碱对DPPH自由基清除作用较强，抗氧化活性显现浓度依赖性。结论 化合物3、5、8、11、16均为首次从该植物中分离得到。化合物5、16抗氧化活性高，可能是该植物主要的抗炎活性成分。

Study on alkaloids from Corydalis saxicola and their anti-oxidative activities

Objective To study the alkaloids from Corydalis saxicola and their anti-oxidative activities. Methods The alkaloids were separated and purified by various column chromatography and identified according to their spectral analyses. The anti-oxidation activities were investigated on DPPH radical scavenging assay. Results Sixteen compounds were obtained and identified as cavidine (1), stylopine (2), canadine (3), tetrahydropalmatine (4), cheilanthifoline (5), scoulerine (6), protopine (7), dehydrocheilanthifoline (8), dehydroisoapocavidine (9), berberine (10), dehydrodiscretamine (11), chelerythrine (12), dehydrocavidine (13), corypalline (14), isocorydine (15), and pallidine (16). The alkaloids from C. saxicla were measured by the model of scavenging the stable DPPH radical, which showed a concentration dependent scavenging effect. Conclusion Compounds 3, 5, 8, 11, and 16 are isolated from C. saxicola for the first time. Compounds 5 and 16 show the strong anti-oxidative activities.