石榴皮鞣质改善大鼠肾纤维化作用研究

目的 研究石榴皮鞣质对肾纤维化大鼠肾功能的改善作用。方法 将SD大鼠随机分为5组，假手术组，模型组，缬沙坦组，石榴皮鞣质高剂量和低剂量（60、20 mg/kg，按鞣质计）组。除了假手术组，其余各组采用左侧输尿管结扎方法制备肾纤维化模型，假手术组的输尿管不结扎和剪断。术后当天开始ig给药，分别于术后1、2、3周检测各组大鼠尿液中的24 h尿蛋白、Ca²⁺和P³⁺浓度，检测血清中的血肌酐（Scr）和尿素氮（BUN），并进行肾组织HE、Masson染色，从形态学角度考察石榴皮鞣质对肾纤维化大鼠各项指标的影响。结果 HE、Masson染色结果显示，模型组大鼠肾组织受到损伤，左侧肾脏肾纤维化，造模成功；生理指标检测结果显示，左侧输尿管结扎后大鼠尿液的24 h尿蛋白，血清Scr、BUN偏离正常水平，Ca²⁺浓度上调，P³⁺浓度下调。石榴皮鞣质干预后，各项指标均具有恢复正常水平的趋势，石榴皮鞣质对大鼠肾纤维化具有改善作用。结论 石榴皮鞣质具有改善大鼠肾纤维化的作用，并能够调节肾纤维化大鼠机体钙磷代谢的失常。

Study on effect of tannins in Granati Pericarpium on renal fibrosis in rats

Objective To study the improvement of tannins from Granati Pericarpium on renal fibrosis in rat. Methods SD rats were randomly divided into five groups, Sham operation, model, Valsartan, high-dose (60 mg/kg), and low-dose (20 mg/kg) tannins groups. Renal fibrosis model of rats was constructed using left ureter ligation method except the rats in the Sham operation group in which the left side ureter of rats was not ligated or cut. The ig administration started at the day of operation, Urinary protein of 24 h, the concentration of Ca²⁺ and P³⁺ in urine, and the levels of Scr and BUN in serum were detected in 1-3 week after the operation. The staining of HE and Masson to the kidney tissue was carried out to investigate how tannins from Granati Pericarpium could impact the indexes of renal fibrosis in rats on the morphological basis. Results The results of urine and blood test showed that the levels of urine protein, Scr, and BUN were deviated from the normal level. The concentration of Ca²⁺ was increased, while the concentration of P³⁺ was reduced. The staining of HE and Masson showed that the renal tissue was damaged, the left side of kidney was found renal fibrosis, tannins from Granati Pericarpium could improve the renal fibrosis in rats in some extent. Conclusion Tannins from Granati Pericarpium have the great improvement on the renal fibrosis in rats and are able to adjust the disorders of Ca²⁺ and P³⁺ in rats.