胃癌区域淋巴结中CD4~+CD25~+Foxp3~+T水平与细胞因子相关性分析

目的观察胃癌区域淋巴结中CD4~+CD25~+Foxp3~+T细胞(Treg细胞)分布及与外周血白介素(IL)-10和转化生长因子(TGF)-β_1的关系。方法采用流式细胞术检测48例胃癌患者(淋巴结转移26例,无转移22例)区域引流淋巴结中Treg细胞频数;ELISA方法检测外周血血清TGF-β_1和IL-10水平。分析Treg细胞与TGF-β_1、IL-10相关性。结果 Treg细胞在淋巴结转移患者阳性淋巴结中水平为(18.16±2.51)%,显著高于阴性淋巴结(13.51±2.48)%],差异有统计学意义(P0.05),且阴性淋巴结Treg细胞比例显著高于无转移患者淋巴结(9.27±2.16)%],差异有统计学意义(P0.05)。ELISA结果提示淋巴结转移患者外周血TGF-β_1和IL-10水平显著高于无转移患者,差异有统计学意义(P0.05)。胃癌患者引流区淋巴结Treg细胞分布比例与外周血血清TGF-β_1(r=0.73,P0.01)和IL-10水平(r=0.81,P0.01)呈明显正相关。结论胃癌患者区域引流淋巴结中Treg细胞分布与淋巴结转移密切相关,Treg细胞可能通过分泌TGF-β_1和IL-10导致胃癌淋巴结微环境发生免疫抑制,是胃癌发生淋巴结转移的重要因素之一。

The relationship of CD4 + CD25 + Foxp3 + regulated T cells in regional lymph nodes and related cytokines in patients with gastric carcinoma

Objective To observe the distribution of CD4 + CD25 + Foxp3 + T cell (Treg) in different regional lymph nodes of gas‐tric carcinoma and to explore the relationship between the TGF‐β1 ,IL‐10 and the level of Treg .Methods The level of Treg cells in regional draining lymph node from 48 case with gastric carcinoma ,including 26 cases with lymphatic metastasis and 22 cases with‐out lymphatic metastasis ,were detected by flow cytometry .And the levels of TGF‐β1 and IL‐10 in peripheral blood serum were checked by ELISA assay .The correlation of the level of Treg with TGF‐ 1 and IL‐10 were analyzed .Results The proportion of Treg cells in positive regional lymph node in patients with lymph node metastasis (18 .16 ± 2 .51)% ] was significantly higher than that of in negative regional lymph node (13 .51 ± 2 .48)% ] in patients with lymph node metastasis (P< 0 .05) .The proportion of Treg cells in negative regional lymph node in patients with lymph node metastasis was significantly higher than that of in regional lymph node in patients without lymph node metastasis (9 .27 ± 2 .16)% ] (P< 0 .05) .The levels of TGF‐β1 and IL‐10 in peripheral blood serum of patients with lymph node metastasis were both significantly higher than those of patients without lymph node me ‐tastasis (P< 0 .05) .And Treg cells were correlated with TGF‐β1 (r= 0 .73 ,P< 0 .01) and IL‐10 (r= 0 .81 ,P< 0 .01) in peripheral blood serum .Conclusion The level of Treg cells in draining regional lymph node of patients with gastric carcinoma is closely relat ‐ed to lymph node metastasis .It is an important reason which leads to lymph node metastasis of gastric carcinoma that Treg could cause immunosuppression happen in regional lymph nodes microenvironment of gastric carcinoma by secreting TGF‐β1 and IL‐10 .