便秘型肠易激综合征患者的血浆5-HT水平与氧化应激作用的相关性分析

目的分析便秘型肠易激综合征患者的血浆5-羟色胺(5-HT)表达与氧化应激作用的相关性。方法选择78例便秘型肠易激综合征患者作为观察组,78例健康人作为对照组,两组均进行血浆5-HT、脂质过氧化物(LPO)和丙二醛(MDA)的检测,以及空腹血糖(FBG)、血清总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)的检测与相关性分析。结果观察组的血浆MDA浓度(4.46±0.51)nmol/L高于对照组(1.68±0.38)nmol/L,差异有统计学意义(P0.05);观察组LPO浓度(18.67±1.48)nmol/L高于对照组(6.62±1.29)nmol/L,差异有统计学意义(P0.05)。观察组血浆5-HT浓度(1 828.44±300.87)pg/mL高于对照组(1 236.93±289.48)pg/mL,差异有统计学意义(P0.05)。偏相关分析便秘型肠易激综合征的血浆5-HT与MDA(r=0.325,P0.05)、LPO(r=0.432,P0.05)、TC(r=0.357,P0.05)都呈正相关,而与HDL-C(r=-0.385,P0.05)呈负相关;多因素Logistic回归分析显示MDA、LPO、TC与HDL-C都为影响5-HT的独立危险因素(P0.05)。结论便秘型肠易激综合征患者中血浆5-HT呈现高表达状况,同时伴随有MDA与LPO的高表达,且MDA与LPO的表达情况会影响5-HT的表达,可能是便秘型肠易激综合征的病理生理机制之一。

The correlation of plasma 5-HT expression and oxidative stress in patients with constipation-predominant irritable bowel syndrome

Objective To analyze the correlation of plasma 5‐hydroxytryptamine (5‐HT ) expression and oxidative stress in pa‐tients with constipation‐predominant irritable bowel syndrome .Methods A total of 78 patients with constipation‐predominant irri‐table bowel syndrome were selected as the observation group ,and 78 healthy people were chosen as the control group at the same period .5‐HT ,lipid peroxide (LPO) and malondialdehyde (MDA) were detected as well as fasting blood‐glucose (FBG) ,triglyceride (TG) ,low density lipoprotein cholesterol (LDL‐C) and high density lipoprotein cholesterol (HDL‐C) .The correlation was analyzed by Partial correlation analysis and Logistic regression analysis .Results The level of MDA (4 .46 ± 0 .51)nmol/L and LPO(1 .68 ± 0 .38)nmol/L in the observation group were higher than that of the control group (P<0 .05) .The level of 5‐HT in the observation group (1 828 .44 ± 300 .87)pg/mL was also found to be significantly higher than that of control group (1 236 .93 ± 289 .48)pg/mL (P<0 .05) .Partial correlation analysis showed that the level of 5‐HT were positively correlated with MDA (r=0 .325 ,P<0 .05) , LPO(r=0 .432 ,P<0 .05) and TC(r=0 .357 ,P<0 .05) in the patients with constipation‐predominant irritable bowel syndrome . However ,level of 5‐HT were negatively correlated with HDL‐C(r= -0 .385 ,P<0 .05) .Logistic regression analysis showed that MDA ,LPO ,TC and HDL‐C were the independent risk factors of the 5‐HT(P<0 .05) .Conclusion Patients with constipation‐pre‐dominant irritable bowel syndrome has over expression levels of 5‐HT accompanied with over expression of MDA and LPO .In addi‐tion ,the expression of MDA and LPO can affect the expression of 5‐HT ,which may be the mechanism of pathophysiology in the constipation‐predominant irritable bowel syndrome .