内脂素rs13237989和rs13224516基因多态性与2型糖尿病的相关性研究

目的探讨内脂素rs13237989和rs13224516基因多态性与广东地区2型糖尿病的相关性。方法收集2013年1月至2014年12月广州医科大学附属第二医院收治新诊断为2型糖尿病患者181例为病例组,另选择体检中心健康者180例为对照组,比较两组血清内脂素rs13237989位点C和T基因型与rs13224516位点A和C基因型的分布频率,并对病例组rs13237989和rs13224516位点不同基因型对应的生化指标进行比较,包括胆固醇(CHOL)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)。结果内脂素rs13237989、rs13224516等位基因频率分布符合遗传定律,样本具有群体代表性,两个基因位点在两组间的基因型多态性分布和等位基因频率差异均无统计学意义(P0.05);Logistic回归分析显示,基因多态性与2型糖尿病无相关性(P0.05);病例组不同基因型携带者TG、CHOL、HDL-C、LDL-C水平差异无统计学意义(P0.05)。结论内脂素rs13237989与rs13224516基因多态性与广东地区2型糖尿病的发生无相关性。

Research on correlation between genetic polymorphism of visfatin rs 13237989,rs13224516 and type 2 diabetes mellitus

Objective To explore the relationship between visfatin rs13237989 ,rs13224516 and type 2 diabetes mellitus (T2DM ) in Guangdong .Methods Totally 181 patients with newly diagnosed T 2DM in the second affiliated hospital of Guangzhou medical university from January 2013 to December 2014 were selected as the case group ,meanwhile 180 healthy people were select‐ed as the control group .The distribution frequency of C and T genotype in rs13237989 site as well as A and C genotype in the rs13224516 site were compared between the case group and the control group .In addition ,biochemical indicators ,including choles‐terol (CHOL ) ,triacylglycerol (TG) ,high density lipoprotein cholesterol (HDL‐C) and low density lipoprotein cholesterol (LDL‐C) were compared in different genotypes of rs13237989 and rs13224516 site in case group .Results The allelic genes distributions frequency of rs13237989 and rs13224516 conformed to the laws of genetic inheritance and the samples in the study were representa ‐tive .The differences of distribution frequencies of genetic polymorphism and allelic genes in the case group and control group had no statistical significance (P > 0 .05) .Logistic regression showed that genetic polymorphism were not correlated with T 2DM (P>0 .05) .TG ,CHOL ,HDL‐C and LDL‐C of different genotypes in patients with T 2DM had no statistical significance (P > 0 .05) . Conclusion There are no difference in genetic polymorphism of visfatin rs13237989 and rs13224516 between case group and control group ,and both of the two kinds of genotypes have no correlation with T 2DM in Guangdong .