HPV-DNA与液基制片法在宫颈癌前病变检测中的临床价值研究

目的比较人乳头瘤病毒(HPV)-DNA检测法与液基制片细胞学检测法在宫颈癌前病变诊断中的临床价值。方法将200例新疆医科大学第二附属医院收集的宫颈病变患者作为研究对象,予以HPV-DNA法和液基制片法检测,比较HPV-DNA法和液基制片法在宫颈癌前病变检测中的准确性。结果采用HPV-DNA检测法的检测阳性率为7.5%,液基制片法为8.5%,两组之间比较差异无统计学意义(P0.05)。以宫颈上皮内瘤变(CIN)Ⅰ级为癌前病变的诊断标准,HPV-DNA检测法筛查宫颈癌前病变的符合率为80.0%,液基制片法为41.2%,两组之间比较差异有统计学意义(P0.05)。并且HPV-DNA检测法的灵敏度和特异度均高于液基制片法。受试者工作特征曲线(ROC)统计分析显示,HPV-DNA法、液基制片法和HPVDNA联合液基制片法这3种检测方法的曲线下面积(AUC)分别为0.761,0.715和0.867。结论 HPV-DNA联合液基制片法是宫颈癌前病变检测较为敏感和准确的方法。

Study of clinical value of HPV-DNA and thinprep cytologic test for detecting cervical precancerous lesion

Objective To compare the clinical value of HPV‐DNA detection and thinprep cytologic test (TCT) in the diagnosis of cervical precancerous lesion .Methods A total of 200 patients with cervical lesion in the Second Affiliated Hospital of Xinjiang Medical University served as the research subjects and performed the HPV‐DNA detection and TCT for comparing their accuracy for detecting cervical precancerous lesion .Results The positive detection rate was 7 .5% for HPV‐DNA detection and 8 .5% for TCT ,the difference between them had no statistical significance (P>0 .05) .With the CIN class Ⅰ as the diagnostic standard ,the coincidence rate of HPV‐DNA detection for screening cervical precancerous lesion was 80 .0% ,which of TCT was 41 .2% ,the difference between them had statistical significance(P<0 .05) .The sensitivity and specificity of HPV‐DNA detection were higher than those of TCT .The ROC curve statistical analysis displayed that the area under the curve (AUC) of HPV‐DNA ,TCT and HPV‐DNA combined with TCT was 0 .761 ,0 .715 and 0 .867 respectively .Conclusion HPV‐DNA detection combined with TCT is the relatively sensitive and accurate method for detecting cervical precancerous lesion .