无偿献血者HIV抗体筛查阳性标本确认结果分析

目的：分析无偿献血者抗 HIV抗体筛查阳性标本的确认实验结果，探讨筛查和确认实验结果的关联，为优化检测策略提供依据。方法采用A和B 2种ELISA试剂筛查抗HIV抗体，阳性样本采用WB法进行确认，采用SPSS13．0进行统计分析。结果185例筛查阳性标本确认符合42例（22．70％），筛查与确认结果差异有统计学意义（χ2＝94．07，P＝0．00）；双试剂阳性标本确认符合率95．24％高于单试剂1．40％（χ2＝162．93，P＝0．00）；S／CO值＞6．00的标本确认符合率最高88．89％（χ2＝149．71，P＝0．00）。结论双试剂联合检测可提高确认符合率，S／CO值＞6．00提示确认阳性率高，应认真选择筛查试剂，合理设置“灰区”，规范操作，尽量减少假阳性发生。

Analysis of the confirmation results of positive samples in the HIV antibody screening of unpaid blood donors

Objective Analyze the confirmation results of positive samples in the HIV antibody screening of unpaid blood do‐nors ,and explore the correlation between the results of screening and confirmation tests ,to provide a basis for the optimization of detection .Methods Screening of anti HIV antibodies by A and B two kinds of ELISA reagents ,positive samples were confirmed by WB method ,and all data were analyzed by SPSS13 .0 statistics software .Results 185 cases of screening positive specimens were confirmed to conform to 42 cases(22 .70% ) .There was a significant difference between the results of screening and confirmation (χ2=94 .07 ,P=0 .00) .Positive samples confirmation coincidence rate detected by two kinds of reagents was higher than single reagent (95 .24% vs1 .40% ,χ2 =162 .93 ,P=0 .00) .Positive samples confirmation coincidence rate in group of S/CO>6 .00 was the highest (88 .89% ,χ2 =149 .71 ,P=0 .00) .Conclusion Detection of two kinds of reagents can improve the confirmation coincidence rate .S/CO value > 6 indicates that the positive rate is high .Laboratory should carefully select screening reagents ,and set“gray area” rea‐sonable ,standardize operations ,so as to reduce the occurrence of false positive .