葛根素对骨性关节炎模型兔的作用机制研究

目的探讨葛根素治疗对骨性关节炎模型兔的作用机制。方法将60只新西兰兔随机分为正常组、模型组、葛根素治疗组,每组20只。正常组不做任何处理,模型组制作膝关节骨性关节炎模型,葛根素治疗组于建模后2周开始用葛根素注射液治疗。在治疗后第6、10周检测各研究组血清和关节液一氧化氮(NO)、诱导型一氧化氮合酶(iNOs)、超氧化物歧化酶(SOD)、丙二醛(MDA)水平。结果第6周时,模型组关节滑液和血清NO、iNOS、MDA水平较正常组显著升高,SOD水平显著降低(P0.05);葛根素治疗组NO、iNOS和SOD水平变化不明显(P0.05),MDA水平显著升高(P0.05)。第10周时,与正常组相比,模型组、葛根素治疗组关节滑液和血清NO、iNOS和MDA水平显著升高,SOD水平显著降低(P0.05)。结论葛根素通过改变血液和关节膜液NO、iNOS、SOD、MDA水平,对骨性关节炎起保护作用。

Effect of puerarin on osteoarthritis in rabbit models

Objective To investigate the effective mechanism of puerarin on osteoarthritis in rabbit models .Methods Sixty New Zealand rabbits were randomly divided into normal group ,model group and puerarin treatment group ,with 20 rabbits in each group . No treatment was performed in rabbit of normal group .Knee osteoarthritis models were established in rabbits of model group .Two weeks after establishment ,puerarin were used to treat rabbit models .At the 6th and 10th week after treatment ,levels of nitrogen monoxidum(NO) ,inducible nitricoxide synthase(iNOS) ,superoxide dismutase(SOD) and malonaldehyde(MDA) in serum and syn‐ovial fluid were measured .Results At the 6th week ,compared with normal group ,NO ,iNOs and MDA levels in synovial fluid and serum of model group were increased significantly ,and SOD level was decreased significantly(P<0 .05) .While the changes of NO , iNOS and SOD levels in puerarin treatment group were not significant(P>0 .05) ,and MDA level was increased significantly(P<0 .05) .At the 10th week ,compared with normal group ,NO ,iNOs and MDA levels in synovial fluid and serum of model group and treatment group were increased significantly ,and SOD level was decreased significantly(P<0 .05) .Conclusion Puerarin could be with protective effects on osteoarthritis by changing levels of NO ,iNOS ,SOD and MDA .