免疫调节性细胞及其效应分子在CITP免疫功能紊乱中的作用研究

目的探讨调节性T细胞(Treg)、调节性B细胞(Breg)、辅助性T细胞17(Th17)、辅助性T细胞22(Th22)及其相关细胞因子的变化在慢性特发性血小板减少性紫癜(CITP)患者免疫功能紊乱中的作用。方法选择CITP患者40例、健康者40例,分离外周血单个核细胞,采用流式细胞术检测Treg、Breg、Th17、Th22细胞数量与比例,采用酶联免疫吸附法检测血浆中白细胞介素10(IL-10)、转化生长因子β1(TGF-β1)、白细胞介素17(IL-17)、白细胞介素(IL-22)水平,计算IL-17/IL-10、IL-22/TGF-β1比值。比较CITP患者、健康者各指标检测结果。结果 CITP患者Th17、Th22细胞及IL-17、IL-22水平高于健康者,Treg、Breg细胞及IL-10、TGF-β1水平低于健康者(P0.05)。CITP患者Th17/Treg、Th22/Breg、IL-17/IL-10、IL-22/TGF-β1比值水平均高于健康者(P0.05)。结论 Th17、Treg、Th22、Breg细胞水平异常及IL-17/IL-10、IL-22/TGF-β1比值变化可能与CITP患者免疫功能紊乱密切相关。

Effect of immunosuppressive celsl and related effective moleculars on the immune disorder in patients with CITP

Objective To study the effect of T helper cells 17(Th17) ,T helper cells 22(Th22) ,regulatory T cells(Treg) ,regula‐tory B cells(Breg)and related cytokines on immune disorder in patients with chronic idiopathic thrombocytopenic purpura(CITP) . Methods A total of 40 CITP patients and 40 healthy subjects were enrolled and detected for levels of Treg ,Breg ,Th17 ,Th22 ,and levels of interleukin‐17(IL‐17) ,interleukin‐22(IL‐22) ,interleukin‐10(IL‐10)and transforming growth factorβ1(TGF‐β1) .The rati‐os of IL‐17/IL‐10 and IL‐22/TGF‐β1 were analysed .Results Levels of Th17 ,Th22 ,IL‐17 and IL‐22 in CITP patients were signifi‐cantly higher than healthy subjects ,but levels of Treg ,Breg ,IL‐10 and TGF‐β1 were lower(P<0 .05) ,The ratios of Th17/Treg , Th22/Breg ,IL‐17/IL‐10 and IL‐22/TGF‐β1 in CITP patients were higher than healthy subjects(P<0 .05) .Conclusion Abnormal‐ity of levels of Th17 ,Th22 ,Treg ,Breg ,and changes of IL‐17/IL‐10 and IL‐22/TGF‐β1 ratios might play an important role in the immune diorder in CITP patients .