高效液相色谱法测定人血清中司帕沙星药物浓度

目的建立测定人血浆中司帕沙星药物浓度的方法。方法采用二氯甲烷萃取，高效液相色谱法测定。色谱柱：GeminiC18（250mm×4．6mm，5μml流动相：0．01mol·L^-1，磷酸二氢钾-三乙胺（磷酸调PH=3．0）-甲醇（34．8：0．2：65）；流速：1．2mL·min^-1；紫外检测器：波长292nm，柱温30℃。结果司帕沙星的保留时间为9．6min，定量线性范围：0．016。2．000μg·ml^-1，方法回收率〉90％（n=5），日内、日间RSD〈10％（n=5）。结论本方法快速，定量准确，可用于血药浓度测定及人体药代动力学研究。

Determination of the content of Sparfloxacin in human plasma by HPLC

Objective To determine the Sparfloxacin concentration in human plasma. Methods The plasma sample was extracted by dichlormethane and determined by HPLC ,the stationary phase was Gemini C18 (250 mm×4.6 mm, 5 μm), the mobile phase consisted of potassium dihydrogen phosphate-triethylamine-methanol. The flow rate was 1.2 ml·min-1, ultraviolet detector was performed with wavelength detector at 292 nm. Results The retention time of Sparfloxaein was about 9.6 min, and the linear range of quantity was 0.016～2.000 μg·ml-1. The recoveries of method were more than 90%(n=5), RSD<10 % (n=5). Conclusions This method is rapid and accurate, it can be used for the content determination of the plasma Sparfloxacin and the study of pharmacokinetics.