| 用PCR技术同时检测微量血痕的种属来源及其DNA多态性 |
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| 引用本文： | 郭俊明,罗超权,杨英浩,伍新尧,刘煦文,罗健扬. 用PCR技术同时检测微量血痕的种属来源及其DNA多态性[J]. 中山大学学报(医学科学版), 1992, 0(4) |
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| 作者姓名： | 郭俊明 罗超权 杨英浩 伍新尧 刘煦文 罗健扬 |
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| 作者单位： | 中山医科大学生物化学教研室(郭俊明，罗超权，杨英浩，刘煦文)，中山医科大学法医系物证教研室(伍新尧)，中山医科大学生物化学教研室(罗健扬) |
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| 摘 要： | 应用聚合酶链反应技术扩增血痕DNA,根据扩增产物中有无aPoB基因的数量可变重复序列(VNTR)及其扩增片段的大小即可同时确定其种属特异性和DNA的多态性。实验结果显示人血痕可见扩增带并具有多态性,而猴猪狗猫鸡血痕无扩增带。
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| 关 键 词： | 聚合酶链反应 血痕 种属鉴定 DNA多态性 |
SIMULTANEOUS DETERMINATION OF DNA SPECIES ORIGIN AND POLYMORPHISIMS IN TRACE BLOODSTAIN WITH PCR |
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| Guo Junming Luo Chaoquan Yang Yinghao Wu Xinyao Liu Xunwen Luo Jianyang. SIMULTANEOUS DETERMINATION OF DNA SPECIES ORIGIN AND POLYMORPHISIMS IN TRACE BLOODSTAIN WITH PCR[J]. Journal of Sun Yatsen University(Medical Sciences), 1992, 0(4) |
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| Authors: | Guo Junming Luo Chaoquan Yang Yinghao Wu Xinyao Liu Xunwen Luo Jianyang |
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| Affiliation: | Department of Biochemistry Department of Forensic Biology |
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| Abstract: | How to use trace bloodstain to obtain more information is one of the most important methods in individual identification in forensic biology. Using the polymerase chain reaction (PCR) with apoB oligonucleotide primers simultaneously determining both the species origin and DNA polymorphisims in a same trace bloodstain are reported. The results demonstrate that the specific DNA fragments and polymorphisims were observed with human bloodstain DNA as the template, but not with the DNA from monkey, pig, dog, cat, and chicken bloodstains. As a result, we found a potentially reliable method for species and individual identification of a trace bloodstain in forensic medicine. |
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| Keywords: | polymerase chain reaction(PCR) bloodstain species origin DNA polymorphisims |
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