| 人精子膜抗原的提纯及分析 |
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| 引用本文： | 沙国柱,黄宇烽,徐建平.人精子膜抗原的提纯及分析[J].中华男科学杂志,1999(3). |
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| 作者姓名： | 沙国柱 黄宇烽 徐建平 |
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| 作者单位： | 解放军肾脏病研究所(沙国柱)，南京军区南京总医院生殖遗传研究室!南京210002(黄宇烽，徐建平) |
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| 摘 要： | 目的：提纯并分析人精子膜抗原。 方法：用表面活性剂 Ｔｒｉｔｏｎ Ｘ １００ 及脱氧胆酸钠（ Ｄ Ｏ Ｃ）的 Ｔ Ｄ Ｏ Ｃ缓冲液提取人精子膜抗原，并与超声法及反复冻融法所获精子抗原通过 Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ法分析比较其成分。 结果： Ｔ Ｄ Ｏ Ｃ缓冲液提取的人精子膜抗原种类丰富，考马斯亮蓝染色见 ２３ 条蛋白（多肽）带，明显优于超声法或反复冻融法所提的抗原。 结论： Ｔ Ｄ Ｏ Ｃ缓冲液所提取的精子膜抗原种类丰富，为进一步研究精子膜抗原及其抗体提供了条件。
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| 关 键 词： | 精子 膜抗原 抗原提取 表面活性剂 |
Extraction and analysis of sperm membrane antigens |
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| Sha Guozhu,Huang Yufeng,Xu Jianping.Extraction and analysis of sperm membrane antigens[J].National Journal of Andrology,1999(3). |
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| Authors: | Sha Guozhu Huang Yufeng Xu Jianping |
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| Abstract: | Objectives:To extracte and analyse sperm membrane antigens. Methods: Sperm membrane antigens were extracted with TDOC buffer containing detergent Triton X 100 and deoxycholate and then separated by reducing SDS PAGE. Results: The result shows that it were rich in kinds of membrane antigens containing 23 bands coloured by Commassie brilliant blue R250,and were better than those extracted with supersonic or repeated freezing thawing method. Conclusions: Sperm membrane antigens could be extracted with the buffer containing detergent Triton X 100 and deoxycholate and the antigens kinds were rich. |
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| Keywords: | Sperm Membrane antigen Antigen extraction Detergent |
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