气相色谱法同时测定醒脑静注射液中麝香酮、龙脑、樟脑、异龙脑的含量

目的:建立毛细管气相色谱法同时测定醒脑静注射液中麝香酮、龙脑、樟脑、异龙脑的含量.方法:采用气相色谱法,DB-FFAP(0.1 μm ×0.53 mm ×30 m)毛细管色谱柱分离测定,FID检测器.进样口温度230℃.检测器温度240℃.用氮气作为载气,流速2.5 mL·min-1;色谱柱采用梯度升温,开始温度85℃,以5℃· min-1的升温速率升至185℃,以20℃·min -1的升温速率升至215℃,以1℃·min-1的升温速率升至230℃,保持5 min.分流比15∶1,进样量1μL,外标法定量.结果:麝香酮、龙脑、樟脑、异龙脑在同一色谱条件下获得分离,麝香酮、龙脑、樟脑、异龙脑在测定范围内呈良好线性关系,回收率符合要求.结论:方法简便、灵敏、准确,分离度、重复性好.

Simultaneous Determination Muscone,Borneol, Camphor and Isoborneol in Xingnaojing Injection by GC

Objective:To establish the simultaneous determination of muscone,borneol,camphor and isoborneol in Xingnaojing Injection.Method: The GC system consisted of capillary column,DB-FFAP-30M;FID as the detector.The column temperature was programmed from 85 ℃ to 185 ℃ at 5 ℃·min-1,then to 215 ℃ at 20 ℃·min-1,to 230 ℃ at 1 ℃·min-1(holding 5 minutes).The inlet temperature was 230 ℃.The detect temperature was 240 ℃.Nitrogen was used as carrying gas(2.5 mL·min-1).The split ratio was 15∶1;injection volume was 1 μL.The external standard method was employed.Result: Muscone,borneol,camphor and isoborneol in Xingnaojing Injection have been separated well under the same chromatographic condition.Muscone,borneol,camphor and isoborneol are in the determination of a good linear relationship within the scope.Recovery rate of the determination method all corresponded to the experimental standard.Conclusion: The method is simple,sensitive,accurate,separable and reproducible,and can be used to control the quality of Xingnaojing Injection.