健脾口服液对脾虚证小鼠胃肠运动功能及胃肠激素分泌的影响

目的:研究健脾口服液对脾虚小鼠胃肠运动功能及胃肠激素分泌的影响,探讨健脾口服液调节胃肠运动功能的机制.方法:60只昆明种小鼠,随机分为6组即健脾口服液高、中、低剂量组、阳性药对照组、模型对照组、正常对照组,每组10只；健脾口服液高、中、低剂量组给药量分别为22.88,11.44,5.72 g·kg-1；启脾口服液为8 mL· kg-1；正常组及模型组给以等体积生理盐水,每天ig给药1次,造模14 d后开始用药,持续给药7d.观察健脾口服液对脾虚小鼠胃肠运动功能及胃泌素、血管活性肽含量的影响.结果:健脾口服液各剂量组均可不同程度的提高脾虚模型小鼠胃甲基橙残留率,降低脾虚模型小鼠小肠内容物推进率,差异有显著性意义；健脾口服液各剂量组均可不同程度的提高脾虚模型小鼠血浆中胃泌素(GAS)、降低血管活性肽(VIP)含量,差异有显著性意义.结论:健脾口服液可以改善脾虚泄泻小鼠胃排空功能和小肠推进功能,提高胃泌素和降低血管活性肽含量可能是治疗脾虚证的作用机制之一.

Effect of Jianpi Oral Liquid on Gastrointestinal Motilityand Gastrointestinal Hormones in Mice Withsyndrome of Spleen-deficiency

Objective: To study the effect of Jianpi oral liquid (JPOL )on gastrointestinal motility, gastrointestinal hormones in mice with syndrome of spleen-deficiency to explore the mechanism. Method: Sixty mice were randomly divided into six groups: normal group,model group,low dose JPOL group, middle dose JPOL group, high dose JPOL group and positive control group (10 each group, including half male and half female ). The normal group and model group were given physiological saline of 15 mL·kg^-1; while JPOL groups were administrated with JPOL at dose of 22.88, 11.44, 5.72 g·kg^-1 respectively, and positive control group was given Qipi oral liquid(QPOL) of 8 mL·kg^-1. Observation started from 14 days after modelling and administration of 7 successive days. Gastrointestinal motility, gastrins(GAS) and vasoactive intestinal peptide(VIP) in each group were observed. Result: Different doses of JPOL could improve the function of gastric emptying and inhabit small bowel peristalsis of mices to different extent. The different doses of JPOL could increase the content of GAS and decrease the content of VIP to different extent. Conclusion: The effect of JPOL may result from improving the function of gastric emptying, inhabiting small bowel peristalsis, the mechanism of which may be related to increasing the level of GAS and decreasing the level of VIP.