美洲大蠊醇提取物的木瓜蛋白酶酶解工艺

目的:优选木瓜蛋白酶对美洲大蠊醇提取物的酶解工艺.方法:以水解度为指标,通过单因素和正交试验,分别考察酶解温度、酶解时间、pH、加酶量、底物浓度对木瓜蛋白酶酶解美洲大蠊醇提取物的影响.结果:木瓜蛋白酶酶解美洲大蠊醇提取物的最佳工艺条件为酶解温度55℃,时间3h,pH 7.0,加酶量8400 U·mg-1,底物浓度0.5％.在此条件下,水解度可达9.87％.酶解后的样品相对分子质量变小,相对分子质量分布变宽.结论:优选方法稳定,操作简便,为进一步研究美洲大蠊提供依据和基础.

Enzymatic Process of Ethanol Extract from Periplaneta americana by Papain

Objective:To study on enzymatic technology of ethanol extract from Periplaneta americana by papain and screen out optimum enzymatic technology.Method: Taking degree of hydrolysis as index,effect of enzymatic temperature,enzymatic time,pH,the amount of papain and substrate concentration on papain were investigated by single factor experiment and orthogonal test.Result: Optimum enzymatic technology conditions of papain was determined as follows:enzymatic temperature 55 ℃,enzymatic time 3 h,pH 7.0,the amount of papain 8 400 U·mg-1,substrate concentration 0.5%.Under these conditions,degree of hydrolysis could reach to 9.87%.Molecular weight of sample were smaller and molecular weight distribution were wider after enzymolysis.Conclusion: This optimized method was stable,easy to operate,and provided basis and foundation to further study of P.americana.