钩藤属植物部分品种药材钩藤碱成分的分析

目的:探索钩藤属部分品种钩藤碱成分的含量,为评价这些品种药材质量提供试验依据.方法:应用HPLC测定钩藤属攀茎钩藤、侯钩藤、毛钩藤、倒挂金钩、北越钩藤和华钩藤33批不同产地药材钩藤碱含量.采用Gemini C18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-水(含0.002 mol·L 1三乙胺,用冰乙酸调pH7.5)(64∶36),流速为1.0 mL·min-1,检测波长254 nm.结果:攀茎钩藤和侯钩藤有5批样品可检出钩藤碱,但含量均较低,其余样品均未能检出钩藤碱.结论:试验所用钩藤属不同品种质量有所差异,同一品种质量随着产地不同而异.

The Analysis of Rhynchophylline Contents in Part of Uncaria Schreb. Plant Breeds

Objective:To probe rhynchophylline contents in part of Uncaria Schreb.plant breeds,and further provide experimental basis for evaluating the quality of medicinal materials.Method:Rhynchophylline in Uncaria Sandens,U.rhynchophylloides,U.hirsuta,U.lancifolia,U.homomalla and U.sinensis from 33 different producing areas were determined by HPLC.HPLC column was Gemini C18(4.6 mm×250 mm,5 μm),mobile phase was methanol-0.002 mol·L-1 triethylomine(pH value 7.5,adjusted with acetic acid)(64∶36),flow rate was 1.0 mL·min-1,and detection wavelength was 254 nm.Result: 5 batches of sample in U.Sandens and U.rhynchophylloides contained rhynchophylline,but content of rhynchophylline was low.The others do not contain rhynchophylline.Conclusion: The different breeds of Uncaria Schreb.plant vary in quality,while the quality of the same breed varies in different producing area.