| 白土苓中大泽明素与总黄酮的含量测定 |
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| 引用本文： | 李进冉,张思巨,李琳,海丽娜,苑秀芝,蒯玉花,仰振球.白土苓中大泽明素与总黄酮的含量测定[J].中国实验方剂学杂志,2012,18(8):68-71. |
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| 作者姓名： | 李进冉 张思巨 李琳 海丽娜 苑秀芝 蒯玉花 仰振球 |
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| 作者单位： | 1. 北京振东光明药物研究院有限公司,北京,100120
2. 中国中医科学院中药研究所,北京,100700 |
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| 基金项目： | "重大新药创制"科技重大专项(2008ZX09202-009) |
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| 摘 要： | 目的:考察白土苓药材中大泽明素、总黄酮的含量.方法:分别采用高效液相法和紫外-可见分光光度法对白土苓药材中的大泽明素和总黄酮进行了含量测定.结果:白土苓药材中大泽明素的平均含量为0.48％,在0.148 8 ～0.7441 μg呈良好的线性关系(r =0.999 4),回收率为101.07％,RSD 1.27％;总黄酮的平均含量为0.52％,橙皮苷在3.708～25.956 mg·L-1与吸光度呈良好的线性关系(r =0.999 9),回收率为99.44％,RSD 1.46％.结论:该方法准确、简便,为科学评价白土苓药材的质量提供依据.
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| 关 键 词： | 白土苓 大泽明素 总黄酮 高效液相色谱法 |
| 收稿时间： | 2011/5/30 0:00:00 |
Studies on Content Determination of Macrozamin and Total Flavonoids in Rhizoma Heterosmilacis |
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| LI Jin-ran,ZHANG Si-ju,LI Lin,HAI Li-n,YUAN Xiu-zhi,KUAI Yu-hua and YANG Zhen-qiu.Studies on Content Determination of Macrozamin and Total Flavonoids in Rhizoma Heterosmilacis[J].China Journal of Experimental Traditional Medical Formulae,2012,18(8):68-71. |
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| Authors: | LI Jin-ran ZHANG Si-ju LI Lin HAI Li-n YUAN Xiu-zhi KUAI Yu-hua and YANG Zhen-qiu |
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| Institution: | Beijing Zhendong Guangming Pharmaceutical Institute Co., Ltd, Beijing 100120, China;Institute of Chinese Meteria Medica China Academy of Chinese Medical Sciences, Beijing 100700, China;Beijing Zhendong Guangming Pharmaceutical Institute Co., Ltd, Beijing 100120, China;Beijing Zhendong Guangming Pharmaceutical Institute Co., Ltd, Beijing 100120, China;Beijing Zhendong Guangming Pharmaceutical Institute Co., Ltd, Beijing 100120, China;Beijing Zhendong Guangming Pharmaceutical Institute Co., Ltd, Beijing 100120, China;Beijing Zhendong Guangming Pharmaceutical Institute Co., Ltd, Beijing 100120, China |
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| Abstract: | Objective:To determine the content of macrozamin and total flavonoids in Rhizoma Heterosmilacis.Method: The content of macrozamin and total flavonoids was determined separately by HPLC and ultraviolet spectrophotometry(UV).Result: The average content of macrozamin in Rhizoma Heterosmilacis was 0.48%.The calibration curve of macrozamin showed good linearity in the range of 0.148 8-0.744 1 μg(r=0.999 4),with recovery rate 101.07% and RSD 1.27%.The average content of total flavonoids was 0.52%.The calibration curve of hesperidin showed good linearity in the range of 3.708-25.956 mg·L-1(r=0.999 9),with recovery rate 99.44% and RSD 1.46%.Conclusion: The methods were approved accurate and simple.They could be used to scientifically evaluate Rhizoma Heterosmilacis. |
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| Keywords: | Rhizoma Heterosmilacis macrozamin total flavonoids HPLC |
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