黔产棘茎楤木抗炎作用及部分机制研究

目的:研究黔产棘茎楤木的抗炎作用及部分机制.方法:采用二甲苯诱导小鼠耳肿胀、角叉菜胶诱导小鼠足爪肿胀、棉球诱导小鼠肉芽肿.弗氏完全佐剂诱导大鼠佐剂性关节炎(AA)模型:设正常对照组、模型对照组、阳性药对照组(雷公藤多苷片12 mg·kg-1)、黔产棘茎楤木高、中、低剂量组(剂量分别为3.5,1.75,0.88 g·kg-1)；除正常对照组外,其余各组均采用弗氏完全佐剂足跖sc复制AA模型；各组于造模当天ig给药,连续35 d,采用硝酸还原法检测各组血清中一氧化氮(NO)含量；放免法检测各组血清中白介素-1β( IL-1β)和肿瘤坏死因子-α(TNF-α)含量.结果:黔棘茎楤木高、中剂量组对小鼠耳肿胀、足爪肿胀有明显抑制作用(P＜0.01,P＜0.05)；对小鼠棉球肉芽肿亦有较好的抑制作用(P＜0.05)；并能降低AA大鼠血清中NO,IL-1β,TNF-α含量(P＜0.01,P＜0.05).结论:黔棘茎楤木可能是通过影响炎性介质的释放和NO的生成而发挥抗炎作用的.

The Anti-inflammatory Effect and Mechanism of Aralia echinocaulis in Guizhou

Objective:To study the anti-inflammatory effect and mechanism of Aralia echinocaulis Hand-Mazzin produced in Guizhou.Method: Dimethylbenzene and carrageenin were used to,respectively,to cause ears edema in mice and paw swelling in rats;medical cotton and freund’s complete adjuvant were used to,respectively,reduce granulomatous inflammation in mice and adjuvant arthritis(AA)in rats.Rats were randomly divided into control group,model group,positive drug group(tripterygium wilfordii 12 mg·kg-1),A.echinocaulis high dosage group,middle dosage group,low dosage group(the dosage 3.5,1.75,0.88 g·kg-1).The corresponding drugs were given at the day of modelling for 35 days.Nitric oxide(NO) content in serum was detected by nitrate reductase method and the level of interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α) was measured with radioimmunoassay(RIA).Result: High dosage group and middle dosage group of A.echinocaul were inhibited the ear edema in mice and swelling of paw(P<0.01,P<0.05);meanwhile granuloma inflammation in mice was reduced(P<0.05),the level of NO,IL-1β and TNF-α was decreased(P<0.01,P<0.05).Conclusion:A.echinocaul can restrain inflammatory reaction,the mechanism of which may be related to inhibiting secretion of NO,L-1β and TNF-α