加味柴芍六君颗粒对实验性溃疡性结肠炎大鼠T淋巴细胞亚群及白介素-2的影响

目的:观察加味柴芍六君颗粒对实验性溃疡性结肠炎(UC)大鼠T淋巴细胞亚群及白介素-2(IL-2)的影响。方法:健康Wistar大鼠90只,随机分为正常组,加味柴芍六君颗粒大、中、小剂量组,SASP组以及模型对照组6组,每组15只。除正常组外,余组制作免疫UC大鼠模型。造模后加味柴芍六君颗粒大、中、小剂量组分别予以1,0.5,0.25mg/ml的加味柴芍六君颗粒溶液,SASP组予以67.5mg/mlSASP混悬液,模型对照组及正常组予以0.5%CMC溶液。连续给药5周后处死大鼠,取出结肠,肉眼观察结肠变化,行组织病理学检测。并采用流式细胞仪检测大鼠血T淋巴细胞亚群,以ELISA法检测大鼠IL-2的表达情况。结果:①加味柴芍六君颗粒能较好地降低溃疡指数。②加味柴芍六君颗粒及SASP均能明显提高UC大鼠CD3、CD4、CD8以及IL-2的水平,降低CD4/CD8的水平,与模型组比较,差异有显著性意义(P<0.01或P<0.05);其中中、小剂量组与SASP组作用几乎相当(P>0.05),大剂量组能使UC大鼠各项指标基本恢复至正常水平,与正常组比较无显著性差异(P>0.05),作用优于SASP组(P<0.01或P<0.05)。结论:改善免疫功能可能是加味柴芍六君颗粒治疗UC大鼠的作用机制之一。

Effects of Jiawei Chaishao Liujun Granule on T lymphocyte Subgroups and IL- 2 Level in Rats with Experimental Ulcerative Colitis (UC)

Object:To observe the effects of Jiawei Chaishao Liujun granule on the T lymphocyte subgroups and IL-2 level in rats with experimental UC.Methods:Ninety healthy Wistar rats were randomly divided into six groups(n=15 in each):the normal group,the high dose,mid-dose and low dose groups,the SASP group,and the model control group.With the exception of the normal group,UC was induced in all the rats in the remaining groups by the immunological method.After modeling,the rats in the high dose,mid-dose and low dose groups were given solution of the Jiawei Chaishao Liujun granule at the doses of 1,0.5 and 0.25 mg/ml,respectively while the rats in the SASP group were administered with 67.5 mg/ml SASP suspension.The 0.5% CMC solution was selected for the model control group and the normal group.The course of successive administration lasted five weeks.All the rats were sacrificed to detect any changes in the colon by the gross examination and subjected to the histopathological test at the end of the administration period.The T lymphocyte subgroups were detected by the flow cytometry.The expression level of IL-2 was detected by ELISA.Results:The ulcer index was lower in the Jiawei Chaishao Liujun granule groups than that in the other three groups.Compared to the model control group,the levels of CD3,CD4,CD8 and IL-2 were significantly higher and the level of CD4/CD8 was lower in the Jiawei Chaishao Liujun granule groups and the SASP group.The difference reached statistical significance(P<0.01 or P<0.05).There was no significant difference between the mid-dose,low dose groups and the SASP group(P>0.05).The curative effects of the high dose of Jiawei Chaishao Liujun granule was better than that of SASP(P<0.01 or P<0.05).Every ulcer index of the rats in the high dose group was restored to be within the normal range in comparison with the normal group(P>0.05).Conclusions:One of the action mechanisms of the Jiawei Chaishao Liujun granule in treating the rats with UC might be that it produces curative effects by improving the immunological function.