HPLC测定四季三黄片中5种蒽醌类衍生物的含量

目的:建立四季三黄片中同时测定芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚含量的方法.方法:色谱柱为Agela Venusil XBP-C18柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1％磷酸,梯度洗脱,流速1.0 mL.min-1,检测波长226nm.结果:芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚分别在0.040 ～0.800,0.040～0.800,0.040～0.800,0.040～0.800,0.020～0.400 μg呈良好的线性关系,平均回收率(n=6)分别为97.62％,99.48％,99.72％,96.23％,95.68％,RSD1.62％,1.24％,1.13％,1.19％,1.67％.结论:所建立的定量方法快速简单,方法可靠,可作为四季三黄片的质量控制方法.

Five Anthraquinone Derivatives in Siji Sanghuang Tablets Determined by HPLC

Objective:To develop a method for simultaneous determining aloe emodin,rhein,emodin,chrysophanol and physcion in Siji Sanghuang tablets.Method: The method was conducted on Agela Venusil XBP-C18(4.6 mm×250 mm,5 μm) column.The mobile phase was acetonitrile(A) and 0.1% phosphoric acid(B) with gradient elution.The flow rate was 1.0 mL·min-1 and the detection wavelength was 226 nm.Result: The linear ranges of aloe-emodin,rhein,emodin,chrysophanol and physcione were 0.040-0.800,0.040-0.800,0.040-0.800,0.040-0.800,0.020-0.400 μg respectively,the average recoveries(n=6) were 97.62%(RSD 1.62%),99.48%(RSD 1.24%),99.72%(RSD 1.13%),96.23%(RSD 1.19%),95.68%(RSD 1.67%) respectively.Conclusion: The method is accurate,reliable,repeatable and can be used for the quality control of Siji Sanghuang tablets.