柴胡舒肝丸质量控制方法考察

目的：建立柴胡舒肝丸的鉴别和含量测定方法。方法：采用TLC法对柴胡舒肝丸进行鉴别，采用HPLC法对黄芩、和厚朴酚及厚朴酚进行含量测定，用Phenomenex Gemini C18（250mm×4．6mm，5μm），以乙腈-0．05％的磷酸溶液进行梯度洗脱，流速为1．0ml·min^-1，检测波长为294nm，进样量为20山。结果：薄层色谱图癍点清晰，黄芩苷的线性范围为4．9～98．4μg·ml^-1（r=0．9994），平均回收率为100．6％，RSD为2．7％（n=9）；和厚朴酚的线性范围为3．2～65．9μg·ml^-1（r=0．9999），平均回收率为99．2％，RSD为2．9％（n=9）；厚朴酚的线性范围为3．1～62．4μg·ml^-1（r=0．9999），平均回收率为98．2％，RSD为1．6％（n=9）。结论：方法准确、重复性好，能控制本品的质量。

Study on the Method for Quality Control of Chaihu Shugan Pills

Objective： To establish the qualitative and quantitative methods for quality control of Chaihu Shugan Pills. Method： Chaihu shugan pills were identified by TLC, the baicalin,magnolol and honokiol in Chaihu Shugan pills were determined by HPLC, the separation of three ingredient were performed on a Phenomenex Gemini C18 （250 mm × 4.6 mm,5 μm） with gradient elution, the aceto- nitrile-0.05% phosphoric acid solution was used as mobile phase, the flow rate was 1.0 ml. min^-1, the detection wavelength was 294 nm, the injection volume was 20μl. Result： The spot on the TLC was clear, the linear ranges of haicalin, magnol and honokiol were 4. 9-98.4 μg.ml^- 1 （ r = 0. 999 4 ）. 3.2-65.9 μg. ml^- 1 （ r = 0. 999 9） and 3.1-62.4 μg. ml^- 1 （ r = 0. 999 9 ）, the average recoveries （ n =9） were 100.6% ,99.2% and 98.2% with RSD 2.7% ,2.9% and 1.6% respectively. Conclusion： This methods is accurate and repeatable,it can be used for quality control of Chaihu Shugan Pills.