FRAS1蛋白与非小细胞肺癌脑转移的关系

目的:探讨FRAS1蛋白与非小细胞肺癌(NSCLC)脑转移的关系。方法:采用q PCR检测FRAS1的mRNA在NSCLC脑转移组织和同期原发灶组织中的表达水平,采用SP免疫组化法检测FRAS1蛋白在肺癌组织和癌旁非肿瘤组织的表达水平,以及有脑转移和无脑转移NSCLC原发灶组织中FRAS1蛋白的表达水平。结果:FRAS1 mRNA在肺癌脑转移灶中的表达量是肺癌原发灶中的近10倍,差异具有统计学显著性(P0.05);FRAS1蛋白在肺癌组织内表达,但在癌旁非肿瘤组织内未见表达;FRAS1蛋白在有脑转移NSCLC患者的肺癌组织中表达明显高于无脑转移NSCLC患者的肺癌组织,差异具有统计学显著性(P0.01)。结论:NSCLC组织中FRAS1蛋白表达可能与肺癌发生有关,同时肺癌组织中FRAS1蛋白高表达可能与肺癌脑转移密切相关。

Relationship between FRAS1 protein and brain metastases of NSCLC

AIM: To explore the relationship between FRAS1 protein and brain metastases of non-small cell lung cancer (NSCLC).METHODS: The mRNA expression of FRAS1 in the brain metastatic tumor tissues and primary tumor tissues of NSCLC was detected by qPCR. The protein expression of FRAS1 in the tumor tissues and normal tissues adjacent to tumor tissues of NSCLC was measured by SP method of immunohistochemistry. The protein expression of FRAS1 in NSCLC primary tumor tissues with or without brain metastases was also determined.RESULTS: The mRNA expression of FRAS1 in the brain metastatic zone was nearly 10 times higher than that in the primary tumor tissues, and there was significant difference between the 2 groups (P<0.05). FRAS1 protein was expressed in the NSCLC primary tumor tissues, but was not found in the normal tissues adjacent to primary tumor tissues. The protein expression of FRAS1 in the NSCLC with brain metastases was significantly higher than that without brain metastases (P<0.01).CONCLUSION: FRAS1 protein may be associated with the occurrence of NSCLC. The over-expression of FRAS1 protein may be related to brain metastases with NSCLC.