脑脊液总蛋白、葡萄糖、氯化物和IgG室间质量评价结果调查

目的总结并分析2012—2016年脑脊液生化检测项目的室间质量评价结果,为提高我国脑脊液检测水平提供参考。方法卫生部临床检验中心每年向参评单位发放脑脊液生化检测项目质控品,收集实验室回报的检测值,对回报的结果按照方法学分组进行统计分析,并评价其检测水平。结果对于总蛋白检测,邻苯三酚红钼铬合显色法变异系数(CV)变化不明显,及格率略有上升趋势;染料结合法2012、2014年CV相对较小,及格率最高,2013、2015、2016年及格率较低;比浊法和干化学法CV波动明显。对于氯化物检测,干化学法CV最小,及格率最高。对于葡萄糖检测,CV均较小且呈逐年降低趋势,及格率均较高(平均及格率99.23%)。对于IgG检测,CV为9.2%～16.43%,及格率低于83.6%但呈逐年增高趋势。结论目前我国脑脊液生化项目的质量水平差异较大,开展室间质评项目计划有利于了解我国脑脊液生化检测的整体水平。

Investigation on external quality assessment of total protein, glucose, chloride and IgG in cerebrospinal fluid

Objective To summarize and analyze the results of national external quality assessment(EQA)of cerebrospinal fluid scheme during 2012-2016 and provide meaningful reference and suggestions for improving the level of cerebrospinal fluid test in China. Methods The national center for clinical laboratories(NCCL)distributed samples to the laboratories participate in the cerebrospinal fluid scheme every year, and collected the test results of laboratory returns, and analyzed the results according to the different methods grouping, and evaluated the detection level. Results For the total protein detection, the CV of the pyrogallol red-molybdenum-chromium method was not obvious, and the pass rate was slightly increased. The CV of dye combination method was relatively small in 2012 and 2014, and the pass rate was the highest. 2013, 2015, 2016 the pass rates were low; The turbidimetry and dry chemical method had significant fluctuations in CV. For chloride , the dry chemical method had the lowest CV and the highest pass rate. For glucose , the CV was small and decreased year by year, and the pass rate was higher (average pass rate was 99.23%). For IgG detection, CV was 9.2% to 16.43%, and the pass rate was lower than 83.6%, but it was increasing year by year. Conclusions The present testing performance for cerebrospinal fluid in China is quite variable. With the help of external quality assessment program, the hole testing performance for cerebrospinal fluid detection will be improved in China.