赫赛汀诱导子宫内膜癌Ishikawa细胞凋亡并增强其对化疗的敏感性

目的:研究赫赛汀单独或联合阿霉素(adriamycin,ADR)、顺铂(cisplatin,DDP)及紫杉醇(paclitaxel,PTX)对子宫内膜癌细胞凋亡和化疗敏感性的影响,为临床应用赫赛汀治疗子宫内膜癌提供理论依据。方法:MTT法检测赫赛汀、ADR、DDP及PTX处理子宫内膜癌Ishikawa细胞的IC50。进一步应用1/2 IC50量的赫赛汀与各1/2 IC50量的ADR、DDP及PTX药物联合,流式细胞术检测细胞周期与凋亡变化。结果:赫赛汀抑制子宫内膜癌Ishikawa细胞生长,引起细胞G1期阻滞,诱导细胞凋亡。子宫内膜癌Ishikawa细胞应用赫赛汀、ADR、DDP及PTX的IC50分别为57.12 mg/L、0.572μmol/L、67.4μmol/L和719.5 nmol/L,赫赛汀联合化疗后显著提高各组化疗药的杀伤效果,诱导细胞凋亡,与单纯化疗组相比差异有统计学意义(P0.05)。结论:赫赛汀诱导子宫内膜癌细胞凋亡,并提高子宫内膜癌细胞株对化疗的敏感性。

Mechanism of Herceptin enhancing apoptosis and improving sensitivity to chemotherapy in Ishikawa cells

AIM: To investigate the role of Herceptin in the apoptosis and drug sensitivity of endometrial cancer Ishikawa cells.METHODS: The IC₅₀ values of Herceptin, adriamycin(ADR), cisplatin(DDP) and paclitaxel(PTX) for Ishikawa cells were detected by MTT method. Ishikawa cells were treated with single drug and combined chemotherapy for 24 h, the cell cycle and the apoptosis ratio were determined by flow cytometry.RESULTS: The IC₅₀ values of Herceptin, ADR, DDP and PTX were 57.12 mg/L, 0.572 μmol/L, 67.4 μmol/L and 719.5 nmol/L, respectively. Herceptin significantly enhanced the cytotoxicity of the chemotherapeutic drugs, and increased apoptosis ratio statistically.CONCLUSION: Herceptin enhances the apoptosis-inducing ability of the chemotherapeutic drugs and improves the chemotherapeutic sensitivity in Ishikawa cells.